Fig. 2.
Representative curves showing steady-state kinetic analysis of 7-PeC versus 7-HpC O-dealkylation by CYP2B35. Kinetic data were collected for CYP2B35 as described in the Materials and Methods section. The kcat, KM, and Ki values determined are shown in Supplemental Table 1. Data for 7-PeC (A) display substrate inhibition, whereas data for 7-HpC O-dealkylation (B) were fit to the Michaelis-Menten equation without substrate inhibition. The points and the error bars reflect the mean and the standard deviation, respectively, from three independent experiments.