Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 8 -
  The first zygotic spindle is organized by the sperm centrioles. Maximum intensity projections of z-stacks of oocytes expressing hsEB3-mCherry3 and pmOdf2-mEGFP (not depicted) acquired every 79 s for a total time of 59 min. Oocyte was fertilized in MI and DMSO was added at MII as control.
• Video 3 -
  A single daughter centriole remains in the mature egg. Daughter centrioles are labeled in green by hsCentrobin-mEGFP; the meiotic spindle is labeled in magenta by pmPoc1-mCherry. Maximum-intensity projections of z-stacks acquired every 35 s for a total time of 47 min.
• Video 5 -
  Mother centriole transport does not depend on PBI cytokinesis. Tracked positions of mother and daughter centrioles are marked with green and pink spheres, respectively. See Fig. 3 (F and G) for details. t = 0 is shortly after NEBD. z-Stacks were acquired every 50 s for a total time of 62 min.
• Video 1 -
  Centrioles organize the meiotic spindles in the starfish oocyte. An oocyte injected with pmPoc1-mEGFP, which labels the four centrioles and the spindle microtubules. Maximum intensity projections of z-stacks acquired every 30 s for a total time of 48 min.
• Video 10 -
  When two mother centrioles are artificially retained in the egg, they remain active and participate in formation of a tetrapolar spindle. Maximum-intensity projections of z-stacks of oocytes expressing hsEB3-mCherry3 and pmOdf2-mEGFP (not depicted) acquired every 101 s for a total time of 76 min. Oocyte was fertilized in MI and treated with latB at MI.
• Video 6 -
  Mother centriole transport is dynein-dependent. Centriole positions were tracked in an oocyte expressing hsEB3-mCherry3 and treated with ciliobrevin D at PBI extrusion. Centrioles are marked by green and pink spheres. See Fig. 3 (H and I) for details. t = 0 is shortly after anaphase I. z-Stacks were acquired every 57 s for ∼30 min.
• Video 2 -
  Mother centrioles are extruded into the polar bodies. Mother centrioles are labeled in green by pmOdf2-mEGFP; the meiotic spindle is labeled in magenta by Cy3-tubulin. Maximum-intensity projections of z-stacks acquired every 38 s for a total time of 42 min.
• Video 4 -
  The mother centriole is transported and anchored to the plasma membrane shortly after MI. Centrioles after tracking (mother and daughter centrioles are represented by the green and pink sphere, respectively). Recording was started shortly after PBI extrusion. Video shows a 3D volume rendering of the data overlaid with an isosurface reconstruction of the cell outline (gray). z-Stacks were acquired every 12 s for a total time of 7 min.
• Video 9 -
  When one mother centriole is artificially retained in the egg, it remains active and participates in the formation of a tripolar spindle. Maximum-intensity projections of z-stacks of oocytes expressing hsEB3-mCherry3 and pmOdf2-mEGFP (not depicted) acquired every 47–51 s for a total time of 52 min. Oocyte was fertilized in MI and treated with latB at MII.
• Video 7 -
  Dynamics of microtubule nucleating activities of centrioles during meiosis. Maximum-intensity projections of z-stacks of oocytes expressing pmPoc1-mEGFP (green) and hsEB3-mCherry3 (magenta) acquired every 36 s for a total time of ∼1 h. Oocyte was centrifuged to spatially separate spindle and centrioles.