This PDF file includes:

• Fig. S1. Synthesis of LA-DNA from an amino T7 primer.
• Fig. S2. Binding of monovalent streptavidin and photocleavage of LA-DNA.
  
• Fig. S3. T7 RNA transcription from LA-DNA.
• Fig. S4. Light-activated expression from LA-mCherry DNA in synthetic cells.
  
• Fig. S5. Expression from amine-only mVenus DNA and LA-mVenus DNA in synthetic cells.
  
• Fig. S6. Light-activated transfer of a small-molecule fluorophore between synthetic cells.
  
• Fig. S7. Light-activated expression from LA-mCherry DNA in synthetic tissues.
  
• Fig. S8. Expression from amine-only mVenus DNA in synthetic tissues.
  
• Fig. S9. Tuning the packing of synthetic cells inside synthetic tissues by altering the bilayer size between synthetic cells.
  
• Fig. S10. Restricted microscope illumination of a synthetic tissue.
  
• Fig. S11. LA-mVenus protein expression in a light-patterned pathway.
  
• Fig. S12. Electrical recordings from an L-shaped 2D pathway formed by light patterning of an LA-HL DNA 3D-printed synthetic tissue.
  
• Fig. S13. The three printing maps used to create the 3D printed pathway.

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