Simultaneous Metabolite, Protein, Lipid Extraction (SIMPLEX): A Combinatorial Multimolecular Omics Approach for Systems Biology

Supplemental Data

• Supplementary Figures (.pdf, 473 KB) - Supplementary figures: S1: Recovery protein and metabolites S2: PPARG activation results in shorter chain length and a lower degree of fatty acid unsaturation. S3: Absolute abundance of identified lipids
• Supplementary Tables (.xls, 3.0 MB) - Table S1. Comparison of extraction protocols for metabolites. Table S2. Comparison of extraction protocols for proteins. Table S3a. Phosphoproteomic dataset obtain after applying SIMPLEX Table S3b. Phosphoproteomic dataset obtained as control Table S4. SRM settings and measurement conditions for investigated metabolites. Table S5. Scheduled SRM conditions to monitoring active PPARG signaling. Table S6. Protein changes after PPARG activation compared to the untreated cells. Table S7. Quantified lipids before and after PPARG activation Table S8. Metabolite changes after PPARG activation compared to the untreated cells.