A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) Transgenesis in Zebrafish

Supporting Information for Fuentes et al., 2016

Files in this Data Supplement:

  • Table S1 - Results for the transgenesis rate of the cxcr4b:Lifeact-Citrine; cryaa:dsRed transgene. (.pdf, 461 KB)
  • Table S2 - Results for the transgenesis rate of the cxcr4b:cxcr4b-Kate2-IRES-GFP-CaaX; cryaa:dsRed transgene. (.pdf, 355 KB)
  • Table S3 - Results for the transgenesis rate of the sdf1a:sdf1a-GFP transgene without Tol2 cis-sequences. (.pdf, 383 KB)
  • Table S4 - Results for the transgenesis rate of the sdf1a:sdf1a-GFP; cryaa:dsRed transgene. (.pdf, 377 KB)
  • Table S5 - Results for the transgenesis rate of the sdf1a:sdf1a-3xFlag-4xHA: cryaa:dsRed transgene of injected fish with no fluorescent protein expression in the lens. (.pdf, 369 KB)
  • Table S6 - Results for the transgenesis rate of the sdf1a:sdf1a-3xFlag-4xHA; cryaa:dsRed transgene of injected fish with fluorescent protein expression in the lens. (.pdf, 374 KB)
  • File S1 - This file contains the ImageJ script that was used to quantify the fluorescent intensity in the primordium shown in Figure 2 (.zip, 2 KB)