Biomass fractionation (Table S1), individual plots of the isotopic values of the data shown in Fig 2 (Fig. S1), acetate formation (Fig. S2), acetate concentration vs. isotopic signal of acetate for S. sphaeroides grown on different substrates (Fig. S3), substrate and product formation for T.kivui grown mixotrophically (Fig. S4), concentration changes of substrate glucose, nitrate, and the product acetate over time for M. thermoacetica (Fig. S5), data for T. kivui growth (Fig. S6 and S7), comparison of the linear regression of the measured and calculated δ13C values of the biomass (Fig. S8), acetate concentrations and isotopic signature of the respective acetate samples (Fig. S9), and HPLC-IRMS results (Table S2).
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