Genotype confirmation via PCR amplification of genomic loci (Fig. S1 to S3), mild acid and alkali treatment of outer membrane purifications (Fig. S4), insertional transposon mutagenesis and phenotype analysis of putative sugar-nucleotide synthesis and polymerization genes (Fig. S5), growth analyses (Fig. S6), effects of initial cyanobacterial cell plating density and suppression of impaired growth phenotype on amoeba resistance (Fig. S7), conservation of LPS synthesis genes across the sequenced cyanobacteria (Fig. S8), and mutations impairing nucleotide-sugar production or polymerization that were attempted and their resulting phenotypes (Table S1).
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