Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 4 - Movie 4 RAW cell expressing ITSN-mCh-SspB, iLID-CaaX, and Venus-Lifeact. Local optical activation of Cdc42 generates increased actin polymerization at the leading edge.
- Movie 5 - Movie 5 RAW cell expressing ITSN-mCh-SspB, iLID-CaaX, and Venus-myosin IIA. Local optical activation of Cdc42 at one side of the cell generates a myosin response at the opposite side.
- Movie 6 - Movie 6 RAW cell expressing ITSN-mCh-SspB, iLID-CaaX, and Venus-myosin IIA imaged before (top) and after (bottom) treatment with ROCK inhibitor. ROCK inhibition blocks myosin accumulation at the cell rear, as well as migration. It also results in more pronounced protrusions at the leading edge.
- Movie 1 - Movie 1 RAW cells expressing ITSN-tgRFPt-SspB and iLID-CaaX. Local optical activation (white boxes) of Cdc42 generates directional cell migration.
- Movie 2 - Movie 2 Comparison of migration controlled by optical activation of the G protein coupled blue opsin (top) or Cdc42 (bottom).
- Movie 3 - Movie 3 RAW cell expressing ITSN-mCh-SspB, iLID-CaaX, and Venus-wGBD (Cdc42 activity sensor). Local optical activation results in Venus-wGBD translocation to the membrane in the activated region, directly demonstrating activation of Cdc42.