Glycyrrhizin Protects against Acetaminophen-Induced Acute Liver Injury via Alleviating Tumor Necrosis Factor α–Mediated Apoptosis

Data Supplement

Files in this Data Supplement:

• Supplemental Data -

  Supplemental Table 1 - List of mouse primers

  Supplemental Figure 1 - Time course of GL and GA in only GL-treated mice and histological analyses of APAP or GL/APAP-treated livers

  Supplemental Figure 2 - GA/18β-GA fails to prevent APAP-induced liver injury

  Supplemental Figure 3 - GL, and not GA and 18β-GA, ameliorates APAP or TNF-α/APAPinduced hepatocyte damage

  Supplemental Figure 4 - GL and GA have no significant effect in Cyp2e1, Cyp3a11, and Cyp1a2 expression in mouse primary hepatocytes

  Supplemental Figure 5 - GL fails to rescue APAP-induced GSH depletion in liver

  Supplemental Figure 6 - GL rescues APAP-induced histological injury in both wild-type and Cyp2e1-null mice

  Supplemental Figure 7 - The effect of APAP and GL in Ripk3 signaling

  Supplemental Figure 8 - Intravenous injection of GL at 2 h post APAP challenge fails to combat APAP-induced liver injury, while both multiple injections of GL and single injection of GL via intraperitoneal injection significantly prevent APAP-induced toxicity