Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 1 -
  Ninein depletion increases CAMSAP 2 speckles in the soma and leading process. A rotating 3D z-stack reconstruction of cerebellar granule neurons transfected with either control (first cell) or ninein siRNA (second cell). Individual images were captured in 0.19-µm intervals using the Zen Blue program and were exported using Fiji. For data analysis and discussion related to Video 1, refer to Fig. 2.
• Video 3 -
  Treatment with Ninein siRNA increases fluorescence decay and treatment of ninein-depleted neurons with FCPT rescues the observed increase. Time-lapse images of the leading process of a migratory neurons, treated with Ninein siRNA (first cell) or ninein siRNA + FCPT (second cell). Ninein-depletion results in an increase in fluorescence decay. After application of FCPT, fluorescence decay was rescued, and MT buckling can be observed. Individual frames in each movie were captured every minute for 20 min using the Zen Blue program and exported using Fiji. Frames are projected at a rate of five frames per second. For data analysis and discussion related to Video 3, refer to Fig. 4.
• Video 4 -
  Migration is compromised after partial ninein depletion, with FCPT restoring cell movement and also leading to an increase in MT buckling in the leading process. Phase images of a migratory neuron treated with ninein siRNA were captured every 30 s for 20 min. 50 µM FCPT was washed into the culture dish, and the same cell was imaged for an additional 20 min. FCPT caused the previously stationary neuron to move, with a concomitant increase in MT buckling in the leading process. Individual frames in each movie were captured using the Zen Blue program and exported using Fiji. Frames are first projected at a rate of five frames per second then replayed at 2.5 frames per second for each treatment. For data analysis and discussion related to Video 4, refer to Fig. 6.
• Video 2 -
  Ninein depletion results in a longer leading process and abnormal neuronal migration. Time-lapse images of a migrating granule neuron, treated with control siRNA (first cell) or ninein siRNA (second cell). Ninein-depletion results in a longer leading process that shows abnormal contortions and increased MT sliding events. Individual frames in each movie were captured every minute for 15 min using the Zen Blue program and exported using Fiji. Frames are projected at a rate of four frames per second. For data analysis and discussion related to Video 2, refer to Fig. 3.