The novel RacE-binding protein GflB sharpens Ras activity at the leading edge of migrating cells

Supplemental Materials

This article contains the following supporting material:

• Supplemental Materials
• Movie 4 - Supplementary Movie 4 Developed GFP-GflB/gflB-cells were observedat 30-sec intervals for 15 min using a Leica DMI6000 and a Yokogawa CSU10 spinning disc confocal microscopewith a 63x objective.
• Movie 3 - Supplementary Movie3 Developed GFP-GflB/gflB-cells were placed in a chemoattractant gradient generated by a micropipette that releasescAMPand were observed for 20 min by phase contrast microscopy.
• Movie 2 - Supplementary Movie 2 Developed gflB-cells were placed in a chemoattractant gradient generated by a micropipette that releasescAMPand were observed for 20 min by phase contrast microscopy.
• Movie 1 - Supplementary Movie1 Developed WT cells were placed in a chemoattractant gradient generated by a micropipette that releases cAMP and were observed for 20 min by phase contrast microscopy.