Staufen1 impairs stress granule formation in skeletal muscle cells from myotonic dystrophy type 1 patients

Supplemental Materials

This article contains the following supporting material:

• Supplemental Materials
• Movie 2 - Video 2: Live-cell microscopy showingconcomitant recruitment of Staufen1 and TIA-1 into SGs in Myoblasts. Proliferative C2C12 myoblasts were transfected with TIA-1-mCherry andStaufen1-GFP. Spinning-disk confocal microscopy was used for time-lapse imaging of transfected myoblasts. Transfected cells were treated with 0.5 mMarseniteto induce SG formation and imaged every 5 minutes for 1 hour.
• Movie 1 - Video 1: Live-cell microscopy showingconcomitant recruitment of Staufen1 and TIA-1 into SGs in Myoblasts. Proliferative C2C12 myoblasts were transfected with TIA-1-mCherry andStaufen1-GFP. Spinning-disk confocal microscopy was used for time-lapse imaging of transfected myoblasts. Transfected cells were treated with 0.5 mMarseniteto induce SG formation and imaged every 5 minutes for 1 hour.