The Histone Variant H3.3 Is Enriched at Drosophila Amplicon Origins but Does Not Mark Them for Activation

Supplemental Material for Paranjape and Calvi, 2016

Supplemental Material

Files in this Data Supplement:

  • File S1 - Supplementary materials and methods. (.pdf, 71 KB)
  • Table S1 - p-values for H3.3A-GFP occupancy in stage 10 (S10) follicle cells. (.pdf, 76 KB)
  • Table S2 - p-values for the difference in H3.3A-GFP occupancy with or without expression of the CDK inhibitor Dacapo (Dap) in stage 10 (S10) follicle cells. (.pdf, 76 KB)
  • Table S3 - p-values for H3.3A-GFP occupancy in stages 1-8 (S1-8) and stage 10 (S10) follicle cells. (.pdf, 79 KB)
  • Table S4 - Location of ChIP-qPCR primers. (.pdf, 75 KB)
  • Figure S1 - Quantification of H3-GFP and H3.3A-GFP intensity at the active DAFC-66D amplicon. (.pdf, 273 KB)
  • Figure S2 - Amplification is inhibited by Dacapo. (.pdf, 128 KB)
  • Figure S3 - ORC occupancy at DAFC-66D in stage 1-8 and stage 10 follicle cells. (.pdf, 142 KB)
  • Figure S4 - A deficit of H3.3 induces a vitellogenic checkpoint response. (.pdf, 147 KB)
  • Figure S5 - H3.3 is not essential for genomic DNA replication or developmental amplification. (.pdf, 152 KB)
  • Figure S6 - EdU amplicon focus intensity in H3.3 null animals is similar to wild type. (.pdf, 192 KB)