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Fig. 5. Immunohistochemical localization of Emx proteins in COS-7 cells transfected with Emx1- or Emx2-expressing plasmid, showing that Emx1 is detected by using the anti-Emx1 antibody, and that Emx2 is detected either with the a -Emx2 or with the a -pan-Emx antibody. Emx1 and Emx2 are mainly detected in the nucleus of the transfected COS cells.

Fig. 6. Immunoperoxidase detection of Emx1 in olfactory bulb. Anti-Emx1 antibody labels the nucleus of cells located in the mitral cells layer (MCL), as well as of periglomerular cells (PC). The axonal tracts and glomeruli (G) in the periphery of the bulb are not labeled.

Fig. 7. (A) Verification that eIF4E was retained on 7mGTP beads. Cell extracts from the olfactory mucosa were incubated with the 7mGTP beads, washed, and analyzed by Western blot. Cell extracts from COS-7 cells were used as a positive size control for the presence of eukaryotic translation initiation factor 4E (eIF4E). (B) Cytosolic extracts from olfactory mucosa were immunoprecipitated (IP) with the anti-Emx2 antibody by using protein A Sepharose beads. Lysate and beads were analyzed in Western blot for the presence of eIF4E. eIF4E is coimmunoprecipitated with Emx2.