The Metalloprotease Mpl Supports Listeria monocytogenes Dissemination through Resolution of Membrane Protrusions into Vacuoles

Supplemental material

• Supplemental file 1 -

  Fig. S1. Computer-assisted image analysis. Fig. S2. Graph showing cytosolic motility recorded in cells infected with the wild type or the mpl or the actA_207-238 mutant. Fig. S3. Characterization of the infection foci formed in cells infected with wild-type (10403S) and 3×Flag ActA (10403S actA3×F) bacteria. Fig. S4. Distribution of ActA on the surface of wt and mpl and ΔactA_207-238 mutant bacteria forming protrusions. Table S1. Primers used in this study. Table S2. Bacterial strains and relevant characteristics. Table S3. Plasmids and relevant characteristics.

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