Influence of Niche-Specific Nutrients on Secondary Metabolism in Vibrionaceae

Supplemental material

  • Supplemental file 1 -

    Examples of a plate prepared by spotting the potential producers and the target strain V. anguillarum on chitin medium, and detailed behavior of V. furnissii S0821 over time and on the two media (Fig. S1); UV/Vis spectra of fluvibactin (Fig. S2); MS and MS/MS spectra of fluvibactin (Fig. S3); proposed fragments of fluvibactin (Fig. S4); 1D 1H NMR spectrum of fluvibactin at 800 MHz (Fig. S5); EIC of fluvibactin for S0821 and S1110 grown with and without iron supplementation (Fig. S6); test of the antibacterial activity of ethyl acetate extracts obtained from cultures of V. furnissii S0821 and V. fluvialis S1162 grown in the presence and in the absence of Fe(III) (Fig. S7); EIC of fluvibactin in SPE fractions of S0821 culture (Fig. S8); assessment of the antibacterial activity of ectoine (Fig. S9); amino acid sequences of the putative bacteriocins predicted by Bagel3 based on the analysis of the genomic sequences of V. furnissii S0821 and V. fluvial S1110 (Table S1); MS/MS fragments of fluvibactin (Table S2); NMR assignment for fluvibactin in CD3OD (Table S3).

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