Analysis of sequencing coverage by rarefaction curves (Text S1); rarefaction curves showing the coverage of bacterial OTU richness as a function of sequencing depth (Fig. S1a); conversion of relative abundance into an extrapolated estimation of population level (Text S2); calibration curves to determine bacterial concentration in meat by quantitative PCR (Fig. S1b); mathematical equations used for quantification of bacteria in meat from CT values (Table S1c); quantification of bacteria in spoiled raw pork sausages (Table S1d); relative abundances, obtained from pyrosequencing, as a function of bacterial concentration determined by qPCR (Fig. S1e).
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OTU abundance table (Data Set S1).
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