Structures for each substrate tested, with specific activity shown below (Fig. S1); chain length versus specific activity (Fig. S2); KES23458 gel filtration trace (Fig. S3); KES23458 thermotolerance by residual activity, triplicate replicates of a differential scanning fluorimetry assay of KES23458 in 10 mM phosphate buffer or in a range of buffers, salts, and pHs (Fig. S4); TA crystal (Fig. S5); mass spectrometry data (Fig. S6); RMSD analysis of KES23458 with β-alanine and 12-aminododecanoic acid docked (Fig. S7); specific activities and relative substrate activities for KES23460 (Table S1).
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