##Percent identity matrix - created by Clustal2.1 1: LsAqp12L1 100.00 64.99 65.53 42.19 42.90 44.87 45.45 45.45 2: aquaporin-12A__C096R035_ 64.99 100.00 99.72 45.50 45.78 43.65 42.90 43.27 3: LsAqp12L2 65.53 99.72 100.00 43.16 45.10 44.11 43.45 43.45 4: aquaporin_3__C030R103_ 42.19 45.50 43.16 100.00 99.40 53.55 53.86 55.51 5: LsGlp1_v1 42.90 45.78 45.10 99.40 100.00 54.81 57.33 57.19 6: LsGlp2 44.87 43.65 44.11 53.55 54.81 100.00 77.28 77.28 7: LsGlp3_v1 45.45 42.90 43.45 53.86 57.33 77.28 100.00 95.09 8: LsGlp3_v2 45.45 43.27 43.45 55.51 57.19 77.28 95.09 100.00 ##CLUSTAL O(1.2.1) multiple sequence alignment LsAqp12L1 ------------------------------------------------------------ aquaporin-12A_(C096R035) ------------------------------------------------------------ LsAqp12L2 ------------------------------------------------------------ aquaporin_3_(C030R103) -----------TTATTTAAATAATAATAATGAGAAAAAAATGTGTGAAGTAAATAGTCCT LsGlp1_v1 ------------------------------------------------------------ LsGlp2 ------------------------------------------------------------ LsGlp3_v1 ATGACAAAG--TT--GTCAATTGAACCTACCTTGCAAT-------------CGTCGTCAG LsGlp3_v2 ATGAGCCAGAATAATTCAGATTCCAACA-----ACAAT-------------AATAGTAAT LsAqp12L1 ------------------------------------------------------------ aquaporin-12A_(C096R035) --------------------------------------GGGGGGAGTGATATTTGAGTGC LsAqp12L2 ------------------------------------------------------------ aquaporin_3_(C030R103) GTGTTATATGAAAATGTTAGGATCGCTAGCCCATTCAAGAAGCGAATATTTTTTGTAAGG LsGlp1_v1 ------------------------------------------------------------ LsGlp2 ------------------------------------------------------------ LsGlp3_v1 ACGT---TG-AGAAACCAGTGAAAG-------GATTATTGTATAAATCCTTT-------- LsGlp3_v2 ACGACTATTATTAATGCAAGGAATG-------GATCTAGGGCTAAATATTTTGTTTGGAC LsAqp12L1 ------------------------------------------------------------ aquaporin-12A_(C096R035) TCCT--TCATTAGGAAAGTA-----AGATCAGCGACAGATATA----ATAATATTACATT LsAqp12L2 ------------------------------------------------------------ aquaporin_3_(C030R103) CTCTAAAAATCCCCAAAGTAAATTAAAAATGTCCACTGATCTGGATAAACCTTATCACTC LsGlp1_v1 ----------------------------ATGTCCACTGATCTGGATAAACCTTATCACTC LsGlp2 ------------------------------------------------------------ LsGlp3_v1 -----GACTTTGAAAA-----------AAA---------TTT--AAG-TGA--------- LsGlp3_v2 CCATAAACATTCAGAAGGAGAAGGACAAAA---TAAGGATTTGGAAGCTAC--------- LsAqp12L1 ------------------------------------------------------------ aquaporin-12A_(C096R035) ACACCT--------------------ATATAGGTGCATTAG-TGGATTTAAAATATTTTT LsAqp12L2 ------------------------------------------------------------ aquaporin_3_(C030R103) ACGACTAACAATTAACATTTTTAATAACTCAGGCTCATTTGATGAAGTTCAAAAC---AC LsGlp1_v1 ACGACTAACAATTAACATTTTTAATAACTCAGGCTCATTTGATGAAGTTCAAAAC---AC LsGlp2 ------------------------------------------------------------ LsGlp3_v1 ---------------------------ATCTGGCAAATTAAGAGAATCTAAGCAG---AC LsGlp3_v2 ---------------------------TTCAGATAGAGTAAGAGAATCTAAGCAG---AC LsAqp12L1 ---------------------------------ATGGCTCTTATACCATGCATCT-GTGT aquaporin-12A_(C096R035) ATTTAATAGTTAAAGGTATTTCTGTGCTTCGACATGGCATTCATTCCATGCATTT-GTGT LsAqp12L2 ---------------------------------ATGGCATTCATTCCATGCATTT-GTGT aquaporin_3_(C030R103) AATTAATAGTTT-------TA---------------------AAGAAATCCAATTCGAGA LsGlp1_v1 AATTAATAGTTT-------TA---------------------AAGAAATCCAATTCGAGA LsGlp2 ---------------------------------------------------------ATG LsGlp3_v1 AGGAAAT---TT-------AT---------------------CAGAAG-GCAT---CATG LsGlp3_v2 AGGAAAT---TT-------AT---------------------CAGAAG-GCAT---CATG LsAqp12L1 ATCAACG----TTTTACAT---------TTGTATCACACTGTTCCTGGCAGAGTTTATGA aquaporin-12A_(C096R035) ATCTCTT----GGCTCTTT---------ATGCCTCACTTTGGCCTTTGCTGAACTTCTTC LsAqp12L2 ATCTCTT----GGCTCTTT---------ATGCCTCACTTTGGCCTTTGCTGAACTTCTTC aquaporin_3_(C030R103) ATGACCATCCTTGTTATATTCCTACCCGTCCTCTAAAAGAATTTTTTTCAAAGTTTATAG LsGlp1_v1 ATGACCATCCTTGTTATATTCCTACCCGTCCTCTAAGAGAATTTTTTTCAGAGTTTATAG LsGlp2 ATATTCGACGTTCCACGAGTTC--GTAGCCTTATACGAGTATTCCTTGCTGAATTATTAG LsGlp3_v1 ATGACCAGTGTTCCTAAAGTCC--CAAGTCTTGTACGACAATTTATGGCAGAACTATTGG LsGlp3_v2 ATGACCAGTGTTCCTAAAGTCC--CAAGTCTTGTACGACAATTTATGGCAGAACTATTGG ** * * * * * * LsAqp12L1 GAACATTTTCTAAAGAGAACTTGAGTCCTGGTCTCCT---TCAAACCGGTATTTTAGAAG aquaporin-12A_(C096R035) GCCTAATCATTCATGCCAGTCTCAAGGGTGGAATCCT---TAAAATTGCACTCCTTGAGT LsAqp12L2 GCCTAATCATTCATGCCAGTCTCAAGGGTGGAATCCT---TAAAATCGCACTCCTTGAGT aquaporin_3_(C030R103) GAACTTTGATCTTCATTACAATCGGTATAGGAACTGTAGCTCAAGTTACACTAAGTGG-- LsGlp1_v1 GAACTTTGATCTTCATTACAATCGGTATAGGAACTGTAGCTCAAGTTACACTAAGTGG-- LsGlp2 GAACATTCCTACTCGTGTATCTCGGAGATGGATCCATTGCTCAGACGGTTGTAGGAGG-- LsGlp3_v1 GAACATTTTTAGTGGTATACCTTGGGGATGGAGCTATTGCACAAGTTGTTGTCGGATC-- LsGlp3_v2 GAACATTTTTAGTGGTATACCTTGGGGATGGAGCTATTGCACAAGTTGTTGTCGGATC-- * * * ** * * * LsAqp12L1 CTATTGCAGCAGCTGAGCTTTG-TGGATG-TTCATT----TGAACTCATCATCATTGCTG aquaporin-12A_(C096R035) TTGTTGCCGCGGCAGAGCTTTG-TGGCTG-CGGATT----TGAGCTCGTTATTATTGCTG LsAqp12L2 TTGTTGCCGCGGCAGAGCTTTG-TGGCTG-CGGATT----TGAGCTCGTTATTATTGCTG aquaporin_3_(C030R103) -CATTCT----CAAGAGTCCTGCTGGCACATTTATATCTACAA----------AT----T LsGlp1_v1 -CATTCT----CAAGAGTCCTGCAGGCACATTTATATCTACAA----------AT----T LsGlp2 ------------AAAATCATCT---CCAT-TAATTTTCTCCAACTTCCTTTCCATTGCTC LsGlp3_v1 ------------ACAAACCTCACAGGCTT-TTTTTTTCTCTAATTTCCTTTCAATTGCAC LsGlp3_v2 ------------ACAAACCTCACAGGCTT-TTTTTTTCTCTAATTTCCTTTCAATTGCAC * * * ** LsAqp12L1 ATAATTATGGAGTCTTTTCATATGGTATTTTTCTTTTTTTCCTCACGATTTGGTGGAGTT aquaporin-12A_(C096R035) ATAATTATGGAATCTCTGCCTACGCTATTTGTCTGTTTCTTTTAACAATCTGGTGGGGAA LsAqp12L2 ATAATTATGGAATCTCTGCCTACGCTATTTGTCTGTTTCTTTTAACAATCTGGTGGGGAA aquaporin_3_(C030R103) GGGGGTTTGGATTCACT---ATTATGATTGGAATGATG-------GTGTCAGGTAAAATA LsGlp1_v1 GGGGGTTTGGATTCGCT---ATTATGATTGGAATGATG-------GTGTCAGGTAAAATA LsGlp2 TTGGCTATGGGTTTGCC---CTCACCATTGGGATATTT-------GTGTCAGGTGGCGTC LsGlp3_v1 TCGGCTATGGTTTTGCC---CTAACAATAGGGATTTTT-------GTATCAGGTGGAGTA LsGlp3_v2 TCGGCTATGGTTTTGCC---CTAACAATAGGGATTTTT-------GTATCAGGTGGAGTA * *** * ** * * * *** LsAqp12L1 CCAGATGGAGCACGGAAACAGCTTGCCCTTACACGCATTTTGAAGAATGTTTAATTGGAA aquaporin-12A_(C096R035) CACGTTGGACTGATGAAACAGCTTGCCCCTATACGCACTTTGAGGAATGCCTTCTTGGGC LsAqp12L2 CACGTTGGACTGATGAAACAGCTTGCCCCTATACGCACTTTGAGGAATGCCTTCTTGGGC aquaporin_3_(C030R103) TCTGGAGGTCATATGAATCCAGCTGTGACAATTG---------CCTTTGCTATCTTTGGT LsGlp1_v1 TCTGGAGGTCATATGAATCCAGCTGTGACAATTG---------CCTTTGCTATCTTTGGT LsGlp2 TCAGGTGGACATTTGAATCCTGCCGTCACTCTAG---------CCATGGCATCCATCAAA LsGlp3_v1 TCTGGCGGTCATCTCAATCCAGCGGTTACTTTAG---------CAATGGCCTCAATAAAA LsGlp3_v2 TCTGGCGGTCATCTCAATCCAGCGGTTACTTTAG---------CAATGGCCTCAATAAAA * ** ** * * * * LsAqp12L1 AGAAGACACTAATCG---AAACAGTAG----TGCGAACATTATCTGGAACATTGGG-AGG aquaporin-12A_(C096R035) GGATGAGCTCATTGG---AGACCATAG----TAAGAACTGTCGCTGA-ATAGTTGG-TGG LsAqp12L2 GGATGAGCTCATTGG---AGACCATAG----TAAGAACTGTCGCTGAAATAGTTGG-TGG aquaporin_3_(C030R103) AAACTAAAACCATGGCATAAATGTTTTC---ATTATATGTTCGCCCA-ATATTTGGGAGC LsGlp1_v1 AAACTAAAACCATGGCATAAATGTTTTC---ATTATATGTTCGCCCA-ATATTTGGGAGC LsGlp2 AAAATGA---AATGG---AAACTTGTCCCAATTTACATGATAGCACA-ATATATTGGAGC LsGlp3_v1 AAATGTA---AATGG---AAATGTGTTCCTGTATATATGTTAGCACA-ATATATTGGAGC LsGlp3_v2 AAATGTA---AATGG---AAATGTGTTCCTGTATATATGTTAGCACA-ATATATTGGAGC * * * * * * * * * * * * LsAqp12L1 TGTCATTGTATTTCAATACATCTACCTCCTATGGTCCATTGAATTGACAGAGACTCATGT aquaporin-12A_(C096R035) ACTTTTAGTTTTTAAGTACATTCATTTCCTATGGTCAATCGAATTTACAGAGACCCGTGT LsAqp12L2 ACTTTTAGTTTTTAAGTACATTCATTTCCTATGGTCAATCGAATTTACAGAGACCCATGT aquaporin_3_(C030R103) CTTTTTTGGTT--CGGCACTCG------------TTTATATTACTTACTATGAAGC-TTT LsGlp1_v1 CTTTTTTGGTT--CGGCACTCG------------TTTATATTACTTACTATGAAGC-TTT LsGlp2 GTTCCTGGCAT--CTGGGACTTTATT---CGGCGTGTATAGAGAT-GGGATTATATTTGT LsGlp3_v1 ATTTTTTGCAT--CAGCTATTCTTTT---TGGGGTTTATAGAGAT-GGAATTATGTTGGT LsGlp3_v2 ATTTTTTGCAT--CAGCTATTCTTTT---TGGGGTTTATAGAGAT-GGAATTATGTTGGT * * * * * ** * * * LsAqp12L1 TGGAAGGGCCCATGGTGTCACTTTCA-------ACAATTGTCCCTCTGATCTTAAAGTTT aquaporin-12A_(C096R035) TGGAAGAGCTCACTCAGCATCTTTCA-------ACCACTGTTCCTCTGATCTGCAAGTAC LsAqp12L2 TGGAAGAGCTCACTCAGCATCTTTCA-------ACCACTGTTCCTCTGATCTGCAAGTAC aquaporin_3_(C030R103) TCAAGAAGCCGGTATCAACATTGACACTGCA---AAAATATTCGCAACATACCCTGCTC- LsGlp1_v1 TCAAGAAGCCGGTATCAACATTGACACTGCA---AAAATATTCGCAACATACCCTGCTC- LsGlp2 GGAGGGGGGCTATAACAAAACGGAGAAAACACAAGGGATCTTTGCTTCATATCCGAGTG- LsGlp3_v1 TGAAGGGGGCTACAATAAAACTGTCAAGACGCAAGGGATTTTTGCTTCTTATCCAAGTC- LsGlp3_v2 TGAAGGGGGCTACAATAAAACTGTCAAGACGCAAGGGATTTTTGCTTCTTATCCAAGTC- * * * * * * LsAqp12L1 CCGCTTATGCAGGGG-------------------CCCTTGTAGAGGGGATTTCCACGATG aquaporin-12A_(C096R035) CAGTACTATACGGAG-------------------CCCTGATTGAAGGGGTGGCTACAATG LsAqp12L2 CAGTACTATACGGAG-------------------CCCTGATTGAAGGGGTGGCTACAATG aquaporin_3_(C030R103) -CTCATATTTCGGCTCCAGGAATTG---------TTCTTGACGAATTTGTTGGAACAGCC LsGlp1_v1 -CTCATATTTCGGCTCCAGGAATTG---------TTCTTGACGAATTTGTTGGAACAGCC LsGlp2 -CGGACATTAATGCA---AGTGTTGCGAGTCTCTGCTTTGATCAAATCCTTGCCACAGCA LsGlp3_v1 -CAAACATTAATGCT---AGCATTGCTAGTTTAAGCTTTGATCAAATCCTTGCCACAGCT LsGlp3_v2 -CAAACATTAATGCT---AGCATTGCTAGTTTAAGCTTTGATCAAATCCTTGCCACAGCT * * * * ** LsAqp12L1 CTATGTCGATGTTCATCCAAATTCCTT--TCAGATAA--ACAACCTCAATACTCGTCTGT aquaporin-12A_(C096R035) ATTTGTCGATGCACTTCCAAATTTCTT--TCGGATAA--GAAGCCTCATTATGCTAGCAC LsAqp12L2 ATTTGTCGATGCACTTCCAAATTTCTT--TCGGATAA--GAAGCCTCATTATGCTAGCAC aquaporin_3_(C030R103) --CTTCTATTAATGTCAGTGTGTGCTATAATTGATAAAAAAAATCTGAACATTCCATCCT LsGlp1_v1 --CTTCTATTAATGTCAGTGTGTGCTATAATTGATAAAAAAAATCTGAACATTCCATCCT LsGlp2 --TTATTGATTATAATTATTCTTGCTGTCACAGATGATAAAAATATGAAAATCATATCAA LsGlp3_v1 --TTGTTGATTATCATTATTCTTGCAGTCACAGATGGTAAAAATATGAAAGTCATATCAA LsGlp3_v2 --TTGTTGATTATCATTATTCTTGCAGTCACAGATGGTAAAAATATGAAAGTCATATCAA * * * * *** * * * LsAqp12L1 GATCGATTCTTTTATCG-CTACATTCC------TTGTAGTT----GCCTCTTTTAAT--T aquaporin-12A_(C096R035) TATTGATTCCTTTGTAG-CAACGTCCC------TCGTCATT----CTAGCTTTCGAT--T LsAqp12L2 TATTGATTCCTTTGTAG-CAACGTCCC------TCGTCATT----CTAGCTTTCGAT--T aquaporin_3_(C030R103) TTTTCCATCCATTTTT-------------------------------------------- LsGlp1_v1 TTTTCCATCCATTTTTAATTGGACTCGTGGTATTTGTCATAACAATGAGCTTTAGTTTTA LsGlp2 GTCTTGTTCCTATATCAATTGGATTGGGACTCACTGCCATTCATCTAAGCTTTGGGGTGA LsGlp3_v1 GTCTTGTTCCTCTATCAATTGGATTGGGACTTACTGCCATTCATCTAAGTTTCGGAGTTA LsGlp3_v2 GTCTTGTTCCTCTATCAATTGGATTGGGACTTACTGCCATTCATCTAAGTTTCGGAGTTA ** * * LsAqp12L1 ACTCTGGGGGTTACCTTAATCCTG-----TACTTGCCACGTCTTTAAAATA--------- aquaporin-12A_(C096R035) ACTCTGGTGGCTATTACAACCCTG-----TACTTGCTACCGGACTTAAATA--------- LsAqp12L2 ACTCTGGTGGCTATTACAACCCTG-----TACTTGCTACCGGACTTAAATA--------- aquaporin_3_(C030R103) ------------------------------------------------------------ LsGlp1_v1 ATGCAGGTGCCGCTTTGAACCCAGCGAGAGATTTAAGACCTCGATTATTTACAGCAGTTT LsGlp2 ACGCTGGAACGGCTATCAATCCAGCTAGGGACTTTGCTCCACGATTATTCACGTTCATTG LsGlp3_v1 ATGCAGGAACAGCAATAAACCCAGCAAGGGATTTTTCTCCGCGTTTATTTACAATAATTG LsGlp3_v2 ATGCAGGAACAGCAATAAACCCAGCAAGGGATTTTTCTCCGCGTTTATTTACAATAATTG LsAqp12L1 -CGGATGTAGAGGTCATCCACTAATGGAGCATATATGTGTGTATTGGATTGGAT------ aquaporin-12A_(C096R035) -TGGTTGTAAGGGGCATCCATTGACGGACCATATACTAGTCTACTGGATAGGAG------ LsAqp12L2 -TGGTTGTAAGGGGCATCCATTGACGGACCATATACTAGTCTACTGGATAGGAG------ aquaporin_3_(C030R103) ------------------------------------------------------------ LsGlp1_v1 TTGGCTGGGGACTAGATCCCTTTTCATATGCCAAACATT---TCTGGATGACACCCATTT LsGlp2 TTGGGTATAGAAGTGGTCCCTTTGTGGCTGGAAAACACTTTTTCTGGATCCCTTGGATCC LsGlp3_v1 CTGGCTATGGAAATGAACCACTTACAACTGGGAATTACTTTTTCTGGATACCTTGGATTC LsGlp3_v2 CTGGCTATGGAAATGAACCACTTACAACTGGGAATTACTTTTTCTGGATACCTTGGATTC LsAqp12L1 ---CATCT---CTAGGTGCCTTT------GCTTCCCTCTACGTGTATCCTCTAGTCTTTA aquaporin-12A_(C096R035) ---CATCC---C------------------------------------------------ LsAqp12L2 ---CATCC---CTTGGAGCAATT------GCATCGATTTACATTTATCCACTCCTCTCTG aquaporin_3_(C030R103) ------------------------------------------------------------ LsGlp1_v1 TAGCGACGCATGCAGGGGCTATAATTGGTGTTTTGATATATCAAACTTTT-----ATAGG LsGlp2 ---TTCCTCATGTGGGTGGAGTTCTTGGAGCCTTGATTTATGAGTACATG-----ATTGA LsGlp3_v1 ---TTCCTCATGTTGGTGCTCTTCTAGGGGCTTTTATTTACGAGTATATG-----ATAGA LsGlp3_v2 ---TTCCTCATGTTGGTGCTCTTCTAGGGGCTTTTATTTACGAGTATATG-----ATAGA LsAqp12L1 ACT---ATACTACAAA---AGCGAAAGAAG-AGTAA------------------- aquaporin-12A_(C096R035) ------------------------------------------------------- LsAqp12L2 ACTTCAATTCTAAAAA---ATCCAAAGTTA-ACTAA------------------- aquaporin_3_(C030R103) ------------------------------------------------------- LsGlp1_v1 ATGGCAATTGTCATTAGAAGACCAAAGAACGCCTAATTTGATAATGAATTTATAG LsGlp2 GCTTCATCATCCAATAGATG---TATGA--------------------------- LsGlp3_v1 ACTTCATCATCCAATCGACG---AATGA--------------------------- LsGlp3_v2 ACTTCATCATCCAATCGACG---AATGA---------------------------