A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner

Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 8 -
  Multiple red actin-Tpm filaments glide toward and along the center of green actin-Tpm bundles, propelled by Myo51–Rng8/9 in a dual-color motility assay. The movies were captured at 1 fps. The red actin-Tpm filaments were less motile or static (potentially cross-linked with the green actin-Tpm) at the edge of the bundles but highly motile at the center. Bar, 5 µm. The video is played at 8 fps.
• Video 6 -
  Multiple red actin-Tpm filaments glide toward and along the center of green actin-Tpm bundles, propelled by Myo51–Rng8/9 in a dual-color motility assay. The movies were captured at 1 fps. The red actin-Tpm filaments were less motile or static (potentially cross-linked with the green actin-Tpm) at the edge of the bundles but highly motile at the center. Bar, 5 µm. The video is played at 10 fps.
• Video 9 -
  A three-panel movie of dual-color motility by Myo51 or Myo51– Rng8/9 captured at 1 fps. The green layer of actin or actin-Tpm was adhered to the nitrocellulose-coated coverslip. (A) Myo51, actin. (B) Myo51, actin-Tpm. ( C) Myo51–Rng8/9, actin. Bar, 12 µm. The movie is played at 10 fps.
• Video 5 -
  A red actin-Tpm filament traveled along a green actin-Tpm bundle, propelled by Myo51–Rng8/9 in a dual-color motility assay. The movie was captured at 1 fps. The layer of green actin-Tpm adhered to the nitrocellulose surface formed bundles with Myo51–Rng8/9. Bar, 10 µm. The movie is played at 10 fps.
• Video 2 -
  Multiple Myo51–Rng8/9 motors bound to a Qdot do not move on actin-Tpm tracks. Qdots, green; actin-Tpm, red. Bar, 5 µm. The movie was captured at 20 fps and is played at 20 fps.
• Video 4 -
  A red actin-Tpm filament traveled along a green actin-Tpm bundle, propelled by Myo51–Rng8/9 in a dual-color motility assay. The filament paused and resumed motility on the motility trajectory. The movie was captured at 1 fps. The layer of green actin-Tpm adhered to the nitrocellulose surface formed bundles with Myo51–Rng8/9. Bar, 5 µm. The movie is played at 12 fps.
• Video 1 -
  Negatively stained EM image series showing Myo51 motor domain bends relative to the lever arm upon addition of MgATP. Arrow indicates the position of the bend. Bar, 20 nm. The movie is played at ten frames per second (fps).
• Video 7 -
  Multiple red actin-Tpm filaments glide toward and along the center of green actin-Tpm bundles, propelled by Myo51–Rng8/9 in a dual-color motility assay. The movies were captured at 1 fps. The red actin-Tpm filaments were less motile or static (potentially cross-linked with the green actin-Tpm) at the edge of the bundles but highly motile at the center. Bar, 5 µm. The video is played at 10 fps.
• Video 3 -
  A four-panel movie of actin or actin-Tpm gliding motility by Myo51 or Myo51– Rng8/9 recorded at 1 fps. The myosins were attached to the neutravidin-coated nitrocellulose surface via their C-terminal biotin tag. (A) Myo51, actin. (B) Myo51, actin-Tpm. (C) Myo51–Rng8/9, actin. (D) Myo51–Rng8/9, actin-Tpm. Bar, 12 µm. The movie is played at 8 fps.