Detection and Diversity of Fungal Nitric Oxide Reductase Genes (p450nor) in Agricultural Soils

Supplemental material

• Supplemental file 1 -

  Supplemental materials and methods, stereomicroscopic images of representative members of isolated fungal groups and singletons identified by sequencing of the cloned ITS region from each fungal isolate (Fig. S1), alignment of P450nor sequences (Fig. S2), N₂O production (Fig. S3), NO₃^-, NO₂^-, and N₂O dynamics (Fig. S4), and intron structure of the region amplified by p450nor primer set p450nor394F/p450nor809R in 47 p450nor sequences (Fig. S5).

  PDF, 4.5M

• Supplemental file 2 -

  NCBI protein GI and accession numbers of P450nor sequences used for primer design (Data Set S1).

  XLS, 32K

• Supplemental file 3 -

  Accessory metadata for the fungal isolates from Havana and Urbana soils (Data Set S2).

  XLS, 33K

• Supplemental file 4 -

  The SILVA 18S rRNA gene classification of 37 fungal isolates in Fig. 1 (Data Set S3).

  XLS, 34K

• Supplemental file 5 -

  p450nor amplicon lengths and number of introns in amplified regions using primer set p450nor394F/p450nor809R (Data Set S4).

  XLS, 34K