Isolation of Endohyphal Bacteria from Foliar Ascomycota and In Vitro Establishment of Their Symbiotic Associations

Supplemental material

• Supplemental file 1 -

  PCR analysis confirms presence/absence of endohyphal bacteria in fungi (Fig. S1); log₁₀ colony-forming units calculated for four replicate cultures of Luteibacter sp. 9143 in LB medium after 1:100 dilution from an overnight culture at 0, 24, and 72 hours after inoculation (Fig. S2); parameters of the reintroduction protocol that were manipulated to evaluate factors influencing in vitro resynthesis of the endohyphal symbiosis of Luteibacter sp. 9143 in Pestalotiopsis sp. 9143 (Table S1).

  PDF, 1.2M