Culturing and Characterization of Gut Symbiont Burkholderia spp. from the Southern Chinch Bug, Blissus insularis (Hemiptera: Blissidae)

Supplemental material

  • Supplemental file 1 -

    List of primers used to PCR amplify the COI genes of Blissus insularis and bacterial genes of crypt-associated symbionts (Table S1), the single-nucleotide polymorphisms detected in the universal 16S rRNA gene partial sequences and the BOX-PCR fingerprinting of crypt-associated and cultured counterpart bacteria isolated from Blissus insularis (Table S2), nucleotide sequence accession numbers of MLST and 16S rRNA gene sequences (Table S3), schematic overview of the 16S rRNA gene sequence of the crypt-associated Burkholderia in Blissus insularis (Fig. S1), comparison of phylogenetic trees (Fig. S2), representative BOX-PCR gel (Fig. S3), representative PFGE patterns of 17 cultured Burkholderia isolates generated from Blissus insularis midgut crypts (Fig. S4), and PFGE patterns of representative cultured Burkholderia isolates (Fig. S5).

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