Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites

Supplemental material

  • Supplemental file 1 -

    Groundwater geochemical parameters from the monitoring wells (Table S1); primers (Table S2); qPCR parameters (Table S3); EaCoMT clone sequences retrieved (Table S4); predicted T-RF lengths (Table S5); GenBank accession numbers (Tables S6 and S7); examples of EaCoMT sequences in VC-, ethene-, and propene-assimilating bacteria that are incorrectly annotated as MetE sequences in GenBank (Table S8); EaCoMT sequences found in the MG-RAST metagenomics database by July 2015 (Table S9); examples of EaCoMT sequences in VC-, ethene-, and propene-assimilating bacteria that are incorrectly annotated as MetE sequences in GenBank (Table S10); phylogenetic tree depicting the relationship of deduced EaCoMT sequences from environmental samples in this study, enrichment cultures, and isolates from GenBank (Fig. S1); NMDS ordination (Fig. S2), bulk VC attenuation rate and point VC decay rate calculations (Fig. S3); linear regression of gene abundance and VC and ethene concentrations (Fig. S4); and analysis of potential bias (Fig. S5).

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