Extended materials and methods, schematic representation of a surface indentation experiment on a bacterial biofilm using AFM (Fig. S1), schematic representation of the measuring setup used for macrorheological characterization of a bacterial biofilm (Fig. S2), time distribution of data obtained by AFM nanoindentation (Fig. S3), viscoelastic properties of B. subtilis B-1 biofilms grown in situ (Fig. S4), surface roughness and bulk elasticity of B. subtilis biofilms after ethanol treatment (Fig. S5), and significance analyses (Tables S1 to S4).
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Bubble formation by B. subtilis B-1 biofilms immediately after ethanol application (Movie S1).
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