begin model
########################################################################################################
begin parameters
# molecules
LCK_T 		500
Csk_T		500



##################################
##################################3
on 8.9125e-04 
off1 0.1072
off2 489.7788
off3 6.0256e+05
off4 7.5858e-04
off5 2.5704e+07
off6 645.6542
off7 229.0868
off8 7.5858e+04
off9 0.0011
off10 0.0012
off11 56.2341
off12 478.6301
off13 15.8489
off14 5.6234e+06
off15 2.5119e-11
off16 1.6218

cat1 2.8184e+03
cat2 41.6869
cat3 7.9433e-11
cat4 4.6774e-06
cat5 0.0048
cat6 38.9045
cat7 13.1826
cat8 0.0098
cat9 6.3096e-12
cat10 1.0715e-10
cat11 7.2444e-08
cat12 1.8621e-06
cat13 1.3183e+03
cat14 6.3096e-11
cat15 8.1283e-04
cat16 0.0631

onCSK 6.0256e-04
offCSKUU 1.4791e-06
offCSKPU 0.0447

CSKcat_PU 1.7783e+07
CSKcat_UU 0.0021

end parameters

########################################################################################################

begin molecule types 
LCK(b,Y505~U~P,Y394~U~P)
Csk(b~0~1)

end molecule types

########################################################################################################

begin seed species
LCK(b,Y505~U,Y394~U) 0.985*LCK_T
LCK(b,Y505~P,Y394~U) 0.015*LCK_T
Csk(b~1) Csk_T


end seed species

########################################################################################################

begin reaction rules
#LCK can autophosphorylate on site 394 only is site 505 is unphosphorylated

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~U,Y505~U) <-> LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U!1,Y505~U) on, off1
LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U!1,Y505~U) -> LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~U) cat1

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~U,Y505~U) <-> LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U,Y505~U!1) on, off2
LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U,Y505~U!1) -> LCK(b,Y394~U,Y505~U) + LCK(b,Y394~U,Y505~P) cat2


LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~U) <-> LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~P,Y505~U!1) on, off3
LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~P,Y505~U!1) -> LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~P) cat3

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~U) <-> LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~P,Y505~U) on, off4
LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~P,Y505~U) -> LCK(b,Y394~P,Y505~U) + LCK(b,Y394~P,Y505~U) cat4

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~U) <-> LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~P,Y505~U) on, off5
LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~P,Y505~U) -> LCK(b,Y394~U,Y505~P) + LCK(b,Y394~P,Y505~U) cat5


LCK(b,Y394~U,Y505~U) + LCK(b,Y394~U,Y505~P) <-> LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U!1,Y505~P) on, off6
LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U!1,Y505~P) -> LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~P) cat6

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~U,Y505~P) <-> LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~U,Y505~P) on, off7
LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~U,Y505~P) -> LCK(b,Y394~P,Y505~U) + LCK(b,Y394~U,Y505~P) cat7

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~U,Y505~P) <-> LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~U,Y505~P) on, off8
LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~U,Y505~P) -> LCK(b,Y394~U,Y505~P) + LCK(b,Y394~U,Y505~P) cat8


LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~P) <-> LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~P,Y505~P) on, off13
LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~P,Y505~P) -> LCK(b,Y394~P,Y505~U) + LCK(b,Y394~P,Y505~P) cat13

LCK(b,Y394~U,Y505~U) + LCK(b,Y394~P,Y505~P) <->  LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~P,Y505~P) on, off14
LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~P,Y505~P) -> LCK(b,Y394~U,Y505~P) + LCK(b,Y394~P,Y505~P) cat14

LCK(b,Y394~P,Y505~U) + LCK(b,Y394~P,Y505~U) <-> LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~P,Y505~U) on, off9
LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~P,Y505~U) -> LCK(b,Y394~P,Y505~P) + LCK(b,Y394~P,Y505~U) cat9

LCK(b,Y394~P,Y505~U) + LCK(b,Y394~P,Y505~P) <-> LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~P,Y505~P) on, off15
LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~P,Y505~P) -> LCK(b,Y394~P,Y505~P) + LCK(b,Y394~P,Y505~P) cat15


LCK(b,Y394~P,Y505~U) + LCK(b,Y394~U,Y505~P) <-> LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~U,Y505~P) on, off10
LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~U,Y505~P) -> LCK(b,Y394~P,Y505~P) + LCK(b,Y394~U,Y505~P) cat10

LCK(b,Y394~P,Y505~U) + LCK(b,Y394~U,Y505~P) <-> LCK(b!1,Y394~P,Y505~U).LCK(b,Y394~U!1,Y505~P) on, off11
LCK(b!1,Y394~P,Y505~U).LCK(b,Y394~U!1,Y505~P) -> LCK(b,Y394~P,Y505~U) + LCK(b,Y394~P,Y505~P) cat11


LCK(b,Y394~U,Y505~P) + LCK(b,Y394~U,Y505~P) <-> LCK(b,Y394~U!1,Y505~P).LCK(b!1,Y394~U,Y505~P) on, off12
LCK(b,Y394~U!1,Y505~P).LCK(b!1,Y394~U,Y505~P) -> LCK(b,Y394~P,Y505~P) + LCK(b,Y394~U,Y505~P) cat12


LCK(b,Y394~U,Y505~P) + LCK(b,Y394~P,Y505~P) <-> LCK(b,Y394~U!1,Y505~P).LCK(b!1,Y394~P,Y505~P) on, off16
LCK(b,Y394~U!1,Y505~P).LCK(b!1,Y394~P,Y505~P) -> LCK(b,Y394~P,Y505~P) + LCK(b,Y394~P,Y505~P) cat16

#Csk can phosphorylate LCK505

Csk(b~1) + LCK(b,Y394~P,Y505~U) <-> Csk(b~1!1).LCK(b,Y394~P,Y505~U!1) onCSK, offCSKPU
Csk(b~1!1).LCK(b,Y394~P,Y505~U!1) -> Csk(b~1) + LCK(b,Y394~P,Y505~P) CSKcat_PU

Csk(b~1) + LCK(b,Y394~U,Y505~U) <-> Csk(b~1!1).LCK(b,Y394~U,Y505~U!1) onCSK, offCSKUU
Csk(b~1!1).LCK(b,Y394~U,Y505~U!1) -> Csk(b~1) + LCK(b,Y394~U,Y505~P) CSKcat_UU


end reaction rules

########################################################################################################

begin observables

Molecules LCK_394P	LCK(Y394~P)
Molecules LCK_505P	LCK(Y505~P)

Molecules LCK_UU    LCK(b,Y394~U,Y505~U)
Molecules LCK_PU    LCK(b,Y394~P,Y505~U)
Molecules LCK_UP    LCK(b,Y394~U,Y505~P)
Molecules LCK_PP    LCK(b,Y394~P,Y505~P)
	
Molecules LCK_b1UU    LCK(b!+,Y394~U,Y505~U)
Molecules LCK_b1PU    LCK(b!+,Y394~P,Y505~U)
Molecules LCK_b1UP    LCK(b!+,Y394~U,Y505~P)
Molecules LCK_b1PP    LCK(b!+,Y394~P,Y505~P)

Molecules LCK_bU1U    LCK(b,Y394~U!+,Y505~U)
Molecules LCK_bUU1    LCK(b,Y394~U,Y505~U!+)
Molecules LCK_bPU1    LCK(b,Y394~P,Y505~U!+)
Molecules LCK_bU1P    LCK(b,Y394~U!+,Y505~P)

Molecules b1UU_bU1U    LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U!1,Y505~U)
Molecules b1UU_bUU1    LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U,Y505~U!1)
Molecules b1UU_bPU1    LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~P,Y505~U!1)
Molecules b1PU_bU1U    LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~P,Y505~U)
Molecules b1PU_bUU1    LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~P,Y505~U)
Molecules b1UU_bU1P    LCK(b!1,Y394~U,Y505~U).LCK(b,Y394~U!1,Y505~P)
Molecules b1UP_bU1U    LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~U,Y505~P)
Molecules b1UP_bUU1    LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~U,Y505~P)
Molecules b1PU_bPU1    LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~P,Y505~U)
Molecules b1UP_bPU1    LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~U,Y505~P)
Molecules b1PU_bU1P    LCK(b!1,Y394~P,Y505~U).LCK(b,Y394~U!1,Y505~P)
Molecules b1UP_bU1P    LCK(b,Y394~U!1,Y505~P).LCK(b!1,Y394~U,Y505~P)
Molecules b1PP_bU1U    LCK(b,Y394~U!1,Y505~U).LCK(b!1,Y394~P,Y505~P)
Molecules b1PP_bUU1    LCK(b,Y394~U,Y505~U!1).LCK(b!1,Y394~P,Y505~P)
Molecules b1PP_bPU1    LCK(b,Y394~P,Y505~U!1).LCK(b!1,Y394~P,Y505~P)
Molecules b1PP_bU1P    LCK(b,Y394~U!1,Y505~P).LCK(b!1,Y394~P,Y505~P)

Molecules CSK_bPU1    Csk(b~1!1).LCK(b,Y394~P,Y505~U!1)
Molecules CSK_bUU1    Csk(b~1!1).LCK(b,Y394~U,Y505~U!1)

end observables 

end model

generate_network({overwrite=>1});
writeMfile({});