Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a Cellulose-Grown Coculture for Enhanced Hydrogen Production

Supplemental material

• Supplemental file 1 -

  Gene ontology terms of the differentially expressed genes (DEGs) of Clostridium cellulovorans 743B (Fig. S1) and Rhodopseudomonas palustris CGA009 (Fig. S2) at early, mid-, and late exponential growth phases in cocultures relative to that in monocultures; comparison of log₂ expression ratios of 16 selected differentially regulated genes between the monocultures and cocultures, measured by using transcriptome sequencing and RT-qPCR (Fig. S3); an overview on how the metabolic pathways of C. cellulovorans 743B (Fig. S4) and R. palustris CGA009 (Fig. S5) were regulated at the early, mid-, and late exponential growth phases in cocultures relative to that in monocultures; comparison of H₂ production for cocultures and monocultures of C. cellulovorans 743B with or without adding ammonium (NH₄ ) in the growth media at a cellulose concentration of 3 g/liter; R. palustris CGA009 with NH₄ produced no hydrogen (Fig. S6); comparison of pH, H₂ production, cellulose degradation, and VFAs concentrations for cocultures and monocultures of C. cellulovorans 743B with NH₄ in the growth media at a cellulose concentration of 3 g/liter (Fig. S7).

  PDF, 7.8M

• Supplemental file 2 -

  Differentially expressed genes of Clostridium cellulovorans 743B (Table S1).

  XLSX, 29K

• Supplemental file 3 -

  Differentially expressed genes of Rhodopseudomonas palustris CGA009 (Table S2).

  XLSX, 87K