Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 2 - Video 2: Localization and timing of Rng10-mEGFP (green) with a cell cycle marker SPB protein Sad1-tdTomato (red). The interval is 4 min in time-lapse confocal microscopy (UltraVIEW Vox CSUX1; PerkinElmer). DIC is shown on the left. The maximum intensity projections of the fluorescence images from 11 slices spaced at 0.6 μm at each time point are shown on the right. This video relates to Figure 2C. Display rate: 6 fps.
- Movie 3 - Video 3: Colocalization of Rng10-mEGFP (green) and Rga7-tdTomato (red). The interval is 2 s in time-lapse confocal microscopy (UltraVIEW Vox CSUX1; PerkinElmer). Two cells imaged at a single focal plane for mEGFP (left), tdTomato (middle), and merged (right) channels are shown. This video corresponds to Figure 4C. Display rate: 6 fps.
- Movie 1 - Video 1: Cell lysis caused by rng10Δ at 25°C. The interval is 2 min in time-lapse confocal microscopy (UltraVIEW Vox CSUX1; PerkinElmer). DIC images are shown. Left, no lysis; middle, one daughter cell lysed; right, both daughter cells lysed. This video corresponds to Figure 1C. Display rate: 2 frames per second (fps).