Biodegradation of DCA by isolates EL1, EL4, EL5, and EL8 (Fig. S1), chloride production from DCA by isolates EL1, EL4, and EL5 (Fig. S2), detection of dhlA genes in isolates EL8, EL1, and EL4 by PCR (Fig. S3), detection of dhlB genes in strains EL1, EL4, EL5, and EL8 by hybridization (Fig. S4), alignment of isolate EL4 hypothetical protein upstream of dhlB1 with transposases from the IS110 family (Fig. S5), expression and purification of His-tagged DhlB2 in E.coli BL21(DE3) (Fig. S6), testing the activity of purified 6×His-DhlB2 protein via release of inorganic chloride from chloroacetate (Fig. S7), arrangements of backbone genes (Fig. S8), details of representative pDCA RepA homologs in GenBank (Table S1), and phylogeny of DhlA from Xanthobacter spp. (Fig. S9).
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