Samples collected and analyzed (Table S1); primers and gBlock positive-control sequences used for end-point and quantitative PCR assays and geosmin and MIB synthases obtained from Sanger sequencing (Table S2); comparison of published primer sets for detecting geosmin-producing cyanobacteria and their homology to the Anabaena geosmin synthase gene observed in Cheney Reservoir (Table S3); identification of known and putative NRPS/PKS or terpene biosynthesis pathways in the two main CyanoHAB genera identified in Lake Cheney by using the secondary-metabolite screening platform antiSMASH 2.0 (Table S4); assessment of shotgun metagenome reproducibility based on a pairwise comparison of assembled contig coverage depths from replicate shotgun metagenome sequences obtained from Cheney Reservoir on 21 October 2013 (Fig. S1); rarefaction analysis of microbial taxa delineated to the genus taxonomic level from shotgun metagenomic sequencing of Cheney Reservoir water column samples concentrated on 0.2-µm-pore-size membrane filters (Fig. S2); discrete and continuous monitoring data obtained from the routine monitoring station throughout 2013 (Fig. S3); sequence comparison of MIB synthase genes recovered from water column samples in 2013 and from periphyton attached to the drinking water intake in 2015, relative to an Oscillatoria limosa reference strain (Fig. S4); log-log regression of total microcystins relative to Microcystis cell counts (A), QPCR estimates of toxigenic (mcyE-possessing) Microcystis (B), and microcystin synthetase operon read coverage depth from shotgun metagenome sequencing (C) (Fig. S5).
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