The Type IV Secretion System Effector Protein CirA Stimulates the GTPase Activity of RhoA and Is Required for Virulence in a Mouse Model of Coxiella burnetii Infection

Supplemental material

• Supplemental file 1 -

  Table S1. Bacterial and yeast strains used in this study. Table S2. Plasmids used in the study. Fig. S1. Replication of wild-type RSA439, the cirA::Tn strain, and the complemented mutant in J774A.1 macrophages infected with an MOI of 50. Fig. S2. Localization of endogenous RhoA during C. burnetii infection. Legends for Movies S1 and S2.

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• Supplemental file 2 -

  Movie S1. Time-lapse video of HeLa cells transiently transfected with EGFP-CirA.

  AVI, 542K

• Supplemental file 3 -

  Movie S2. Time-lapse video of HeLa cells transiently transfected with EGFP.

  AVI, 652K