Primary and Secondary Sequence Structure Requirements for Recognition and Discrimination of Target RNAs by Pseudomonas aeruginosa RsmA and RsmF

Supplemental material

  • Supplemental file 1 -

    Tables S1 (Primers), S2 (gBlock gene fragments), and S3 (DNA templates for generation of RNA probes) and Fig. S1 (Length analysis of the top 100 targets for RsmA and RsmF), S2 (OmegaCluster alignment of targets >96 nt selected by RsmF), S3 (WebLogo predictions for positional clusters for RsmF targets >96 nt), S4 and S6 (Predicted Mfold structures for three RsmF targets [S4] and select structures with two GGA sequences [S6]), S5 (RsmA and RsmF binding to targets), S7 and S8 (Native gel electrophoresis and phosphorimaging), and S9 (Experimentally verified sites for RsmA and RsmF)

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