Supplemental materials and methods
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Fig. S1 (Sequence alignment of the forward primer, reverse primer, and probe binding regions for 107 representative mycobacterial species), S2 (Sequence alignment of the forward primer, reverse primer, and probe to the hsp65 gene region for 40 nonmycobacterial species, M. abscessus, M. tuberculosis, and M. leprae), S3 (Relationship between CT values and log numbers of CFU/ml in reaction mixtures spiked with DNA extracts from culture-negative BAL or sputum samples), S4 (Relationship between CT values and log numbers of CFU/ml in reaction mixtures spiked with purified human DNA), and S5 (Relationship between CT values and log numbers of CFU/ml in reaction mixtures spiked with horse blood) and Tables S1 (The 75 mycobacterial species with complete sequence homology at primer and probe binding sites), S2 (In silico evaluation of 14 mycobacterial species that have <100% primer sequence homology but which are amplifiable at annealing temperatures of ≥55.5°C), S3 (Mycobacterial and nonmycobacterial species that require an annealing temperature of ≤55.3°C for amplification), S4 (Details of clinical samples used for assay validation), and S5 (Detection of NTM species from respiratory samples using culturebased and molecular approaches)
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