This PDF file includes:

• Supplementary Materials and Methods
• fig. S1. Representative backbone configurations of the helix bundle motif building block illustrating the variation of the geometric parameters associated with the bundle.
• fig. S2. Side view and top view of the selected low-energy helix bundle motif with the most probable amino acids at the interior sites shown in space-filling representations.
• fig. S3. P422_1 analytical HPLC.
• fig. S4. P422_1_ac analytical HPLC.
• fig. S5. P222_9 analytical HPLC.
• fig. S6. P222_9 sequence (3168 daltons).
• fig. S7. P422_1 sequence (3572 daltons).
• fig. S8. BNDL_1 (3560 daltons).
• fig. S9. P622_6 (3517 daltons).
• fig. S10. P222_9 (0.1 mM) in borate buffer (pH 10) on heating.
• fig. S11. P622_6 (0.1 mM) in phosphate buffer (pH 7) on heating.
• fig. S12. BNDL_1 (0.1 mM) in borate buffer (pH 10) on heating.
• fig. S13. P422_1 (0.1 mM) in borate buffer (pH 10).
• fig. S14. AUC data and analysis of BNDL_1.
• fig. S15. High-magnification TEM of 1.0 mM P222_9 ambiently cooled to room temperature from 80°C at pH 7 (left) and pH 10 (right).
• fig. S16. High-magnification TEM of (left) lattice of P222_9_Ac at pH 7 ambiently cooled to room temperature from 80°C and (right) P422_1_Ac at pH 8 quenched to 40°C from 80°C.
• fig. S17. High-magnification TEM of lattice of P222_9_6Gly at pH 7 ambiently cooled to room temperature from 80°C.
• fig. S18. Putative structure of assembly of P422_1 helix bundles packed with P4 symmetry, which is a local minimum within the structure energy landscape with respect to variation of the unit cell parameter.
• fig. S19. Putative assembly structures of P222_1 assemblies with P2 symmetry located at a local minimum of the structure energy landscape with respect to variation of the unit cell parameters.
• References (39–52)

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