Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 5 - Supplementary Movie 5: In vitro scratch assay for MCF10A monolayers (Control) (Related to Supplementary Figure 3C) In vitro scratch assay for control MCF10A monolayers, transfected with a non-targeting siRNA (34-36hr post-siRNA transfection). Movie acquired at 10min intervals over 24hr, playback at 15fps.
- Movie 6 - Supplementary Movie 6: In vitro scratch assay for MCF10A monolayers (FMNL3 knockdown) (Related to Supplementary Figure 3C) In vitro scratch assay for FMNL3 knockdown MCF10A monolayers (34-36hr post-siRNA transfection). Note the severe perturbation of collective migration and increased dissociation of cells from the migrating fronts. Movie acquired at 10min intervals over 24hr, playback at 15fps.
- Movie 1 - Supplementary Movie 1: Apical junction dynamics in Control vs. SMIFH2-treated monolayers (Related to Figure 2A) E-cadherin-GFP dynamics at the ZA in control (top) and SMIFH2-treated monolayers (bottom). Green arrowheads (bottom panel) highlight junctions that undergo shortening during the period of imaging. Movie acquired at 1min intervals, playback at 6 frames per second (fps).
- Movie 2 - Supplementary Movie 2: Baso-lateral junction dynamics in Control vs. SMIFH2-treated monolayers (Related to Figure 2B) E-cadherin-GFP dynamics at basolateral contacts in control (top) and SMIFH2-treated monolayers (bottom). Note the formation of filopodia in control vs. lamellipodia after SMIFH2 treatment. Movie acquired at 1min intervals, playback at 6fps.
- Movie 3 - Supplementary Movie 3: In vitro scratch assay for Eph4 monolayers (Control) (Related to Figure 4G)In vitro scratch assay for control Eph4 monolayers, transfected with a non-targeting siRNA (20-22hr post-siRNA transfection). Movie acquired at 15min intervals over 12hr, playback at 7fps.
- Movie 7 - Supplementary Movie 7: In vitro scratch assay for Eph4 monolayers (Fmnl3 and mDia1 knockdown) (Related to Figure 4G) In vitro scratch assay for Fmnl3 and mDia1 knockdown Eph4 monolayers (20-22hr post-siRNA transfection). Movie acquired at 10min intervals over 12hr, playback at 7fps.
- Movie 4 - Supplementary Movie 4: In vitro scratch assay for Eph4 monolayers (Fmnl3 knockdown) (Related to Figure 4G) In vitro scratch assay for Fmnl3 knockdown Eph4 monolayers (20-22hr post-siRNA transfection). Note the severe perturbation of collective migration and increased dissociation of cells from the migrating fronts. Movie acquired at 15min intervals over 12hr, playback at 7fps.