PRIMER_TASK=generic PRIMER_MISPRIMING_LIBRARY= PRIMER_MIN_TM=45.0 PRIMER_OPT_TM=55.0 PRIMER_MAX_TM=70.0 PRIMER_MIN_GC=20.0 PRIMER_MAX_GC=100.0 PRIMER_NUM_RETURN=5000 PRIMER_MIN_SIZE=20 PRIMER_OPT_SIZE=30 PRIMER_MAX_SIZE=35 PRIMER_PRODUCT_SIZE_RANGE=100-300 SEQUENCE_ID=TS001 SEQUENCE_TEMPLATE= PRIMER_PICK_LEFT_PRIMER=1 PRIMER_PICK_RIGHT_PRIMER=1 PRIMER_PICK_INTERNAL_OLIGO=1 PRIMER_PICK_ANYWAY=1 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0