A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins

Supplemental Data

• Supplemental Data (.pdf, 138 KB) - Alkyne lipids (A), biotin-based azide detection reagents (B), and fluorescent azide detection reagents (C) used in this study.
• Supplemental Data (.pdf, 236 KB) - Supplementary Information for synthesis of reagents (AP6Btn, AP10Btn, APpic2Btn, and ApicSBDP) and troubleshooting guide for the labeling of alkyne lipids with azide reporters for lipid imaging