Functional Genome Mining for Metabolites Encoded by Large Gene Clusters through Heterologous Expression of a Whole-Genome Bacterial Artificial Chromosome Library in Streptomyces spp.

Supplemental material

• Supplemental file 1 -

  BAC vector pHL921 (Fig. S1); streptothricins produced by S. rochei Sal35 (Fig. S2); determination of BAC insert size by PFGE (Fig. S3); output of the antimicrobial screening in LEXAS (Fig. S4); contigs grouped by PvuII restriction digestion (Fig. S5); heterologous expression and identification of borrelidin (Fig. S6); detection and identification of CDAs produced by S. lividans SBT18/8D1 (Fig. S7); disrupted cda BGC in S. lividans SBT5 and S. lividans SBT18 (Fig. S8); detection of 8D1-1 and 8D1-2 in S. rochei Sal35 and S. coelicolor M1152/8D1 by HPLC (Fig. S9); chiral analysis of Hpg6 and 3-OH-Asn9 in 8D1-1 (Fig. S10: comparison of gene organizations in cda2 and cda pathways from S. rochei Sal35 and S. coelicolor A3(2) (Fig. S11); homology alignment of epimerization domains (Fig. S12); ¹³C (201 MHz) and ¹H (800 MHz) NMR data for 8D1-1 in DMSO-D₆ (Table S1); ¹³C (201 MHz) and ¹H (800 MHz) NMR data for 8D1-2 in DMSO-D₆ in redicated functions of the proteins encoded by the cryptic BGC in contig 4 (Table S3).

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