Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 2 - Video 2. Time-lapse movie of bipolar spindle assembly in a mitotic H2B-eGFP/α-tubulin-mRFP HeLa cell undergoing MT regrowth after nocodazole washout. Video corresponding to the selected still images shown Figure 1B (central top panel). Images were acquired every 3 min by time-lapse epifluorescence microscopy. Tubulin is in green and H2B in red. Time indicates the time after nocodazole washout in hours:minutes. Scale bar =10μm.
- Movie 5 - Video 5. Time-lapse movie of spindle assembly in control and mitotic γ-tubulin-paGFP/α-tubulin-mCherry U2OS cells undergoing MT regrowth. Video corresponding to γ-tubulin-paGFP/α-tubulin-mCherry U2OS cells imaged every 3 minutes after nocodazole washout by time-lapse epifluorescence microscopy. Images were acquired every 3 min. Tubulin is in grey. Time indicates the time after nocodazole washout in hours:minutes. Scale bar =10μm.
- Movie 6 - Video 6. Time-lapse movie of spindle assembly in control and mitotic α-tubulin-GFP LLC-PK1α cells undergoing MT regrowth. Video corresponding to α-tubulin-GFP LLC-PK1α cells imaged every 3 minutes after nocodazole washout by time-lapse epifluorescence microscopy. Images were acquired every 3 min. Tubulin is in grey. Time indicates the time after nocodazole washout in hours:minutes. Scale bar =10μm.
- Movie 4 - Video 4. Time-lapse movie of disorganized spindle assembly in a mitotic H2B-eGFP/α-tubulin-mRFP HeLa cell undergoing MT regrowth after nocodazole washout. Video corresponding to the selected still images shown in Figure 1B (bottom panel). Images were acquired every 3 min by time-lapse epifluorescence microscopy. Tubulin is in green and H2B in red. Time indicates the time after nocodazole washout in hours:minutes. Scale bar =10μm.
- Movie 1 - Video 1. Time-lapse movie of spindle assembly in a control H2B-eGFP/α-tubulin-mRFP HeLa cells. Video corresponding to the selected still images shown in Figure 1B (top panel). Images were acquired every 3 min by time-lapse epifluorescence microscopy. Tubulin is in green and H2B in red. Time indicates the time after nocodazole washout in hours:minutes. Scale bar =10μm.
- Movie 3 - Video 3. Time-lapse movie of tripolar spindle assembly in a mitotic H2B-eGFP/α-tubulin-mRFP HeLa cell undergoing MT regrowth after nocodazole washout. Video corresponding to the selected still images shown in Figure 1B (central bottom panel). Images were acquired every 3 min by time-lapse epifluorescence microscopy. Tubulin is in green and H2B in red. Time indicates the time after nocodazole washout in hours:minutes. Scale bar =10μm.