Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking

Supplemental Data

• Table S1 (.xlsx, 88 KB) - List of proteins and number of unique peptides identified per protein in the SIRT2-EGFP and control isolations
• Table S2 (.xlsx, 97 KB) - List of proteins and amino acid sequence coverages for the proteins identified in the SIRT2-EGFP and control isolations
• Table S3 (.xlsx, 92 KB) - List of SAINT scores for all proteins identified in the SIRT2-EGFP IP
• Table S4 (.xlsx, 19 KB) - Refined list of SIRT2-EGFP interacting proteins that passed SAINT and Crapome filtering
• Table S5 (.xlsx, 14 KB) - List of SIRT2-EGFP interacting proteins following nuclear-cytoplasmic fractionation of primary human fibroblasts
• Table S6 (.xlsx, 346 KB) - Assessment of SILAC label incorporation efficiency in human primary fibroblasts
• Table S7 (.xlsx, 40 KB) - List of SIRT2-EGFP interacting proteins identified in metabolic labeling-based quantification experiment with number of unique peptides and amino acid sequence coverages per identified protein
• Table S8 (.xlsx, 101 KB) - List of SIRT2-EGFP interacting proteins identified in metabolic labeling-based quantification experiment with heavy/total ratios per identified protein
• Table S9 (.xlsx, 317 KB) - List of proteins identified in whole-cell lysates following shRNA-mediated knockdown of SIRT2 or control with number of unique peptides and amino acid sequence coverages indicated
• Table S10 (.xlsx, 718 KB) - Ratios of protein levels identified in whole-cell lysates following shRNA-mediated knockdown of SIRT2 or control
• Table S11 (.xlsx, 22 KB) - List of Gene Ontology Biological Processes annotations associated with proteome changes observed following SIRT2 knockdown