Systems-level Proteomics of Two Ubiquitous Leaf Commensals Reveals Complementary Adaptive Traits for Phyllosphere Colonization

Supplemental Data

• Supplemental Table 1 (.pdf, 435 KB) - Differentially regulated transport proteins of S. melonis Fr1 and M. extorquens PA1 during colonization of the phyllosphere.
• Supplemental Table 2 (.pdf, 427 KB) - Methanol metabolism of M. extorquens PA1 during colonization of the A. thaliana phyllosphere.
• Supplemental Table 3 (.pdf, 566 KB) - Oxalate metabolism of M. extorquens PA1 during colonization of the A. thaliana phyllosphere.
• Supplemental Table 4 (.pdf, 389 KB) - Predicted operon structures containing alkanesulfonate monooxygenase genes
• Supplemental Table 5 (.pdf, 318 KB) - KEGG orthology terms and functional annotation of co-regulated proteins between M. extorquens PA1 and S. melonis Fr1.
• Supplemental Figure 1 (.pdf, 479 KB) - Venn diagrams showing the overlap of proteins contained in the SWATH assay libraries obtained by shotgun proteomics (green: OGE-fractionated samples derived from liquid bacterial cultures in three different growth stages; red: unfractionated samples derived from bacteria grown on plant leaves) as well as of proteins actually detected by SWATH MS in the unfractionated plate- and plant-derived bacterial samples (yellow: plate; blue: plant). OGE, off-gel electrophoresis.
• Supplemental Figure 2 (.pdf, 565 KB) - Central carbon metabolism of M. extorquens PA1 during plant colonization as compared to colonization of minimal media agar plates. Pathways and regulation of respective proteins is indicated. Proteins are numbered and listed in Tab. S1 for more details.
• Supplemental Figure 3 (.pdf, 1.7 MB) - Random field of views of M. extorquens PA1 cells grown on minimal medium agar plates in the dark.
• Supplemental Data 1 (.xlsx, 19.5 MB) - Library peptides
• Supplemental Data 2 (.xlsx, 6.2 MB) - OpenSWATH
• Supplemental Data 3 (.xlsx, 296 KB) - ANOVA
• Supplemental Data 4 (.xlsx, 414 KB) - aLFQ