Runt-related Transcription Factor 1 (RUNX1) Binds to p50 in Macrophages and Enhances TLR4-triggered Inflammation and Septic Shock

Supplemental Data

• supplementary information (.pdf, 766 KB) - Figure S1. The purity of murine PEMs. Figure S2. The protein levels of Runx1 in LPS-stimulated RAW264.7 cells and PEMs. Figure S3. The expression levels of Runx1 in PEMs after stimulation with cytokines. Figure S4. The mRNA expression levels of LPS-dependent genes in PEMs after Runx1 siRNA transfection. Figure S5. The RUNX1 inhibitor Ro 5-3335 reduced IL-6 mRNA levels in PGN-treated PEMs. Figure S6. The RUNX1 inhibitor Ro 5-3335 does not affect p65 phosphorylation and IκBα degradation in LPS-stimulated PEMs. Figure S7. The effect of RUNX1 on the recruitment of p50 to the IL-6 promoter. Figure S8. The effect of RUNX1 on TRIF-dependent cytokines and IRF3 phosphorylation. Figure S9. The specificity of Runx1 siRNA. Supplementary Table 1. The primers used to clone target genes. Supplementary Table 2. siGENOME mouse Runx1 siRNA ?SMARTpool. Supplementary Table 3. Negative control scrambled siRNA Supplementary Table 4. The primers used for Real-time qPCR. Supplementary Table 5. The primers used for qPCR-coupled ChIP analysis.