DNA sequence of the synthetic codon usage-optimized CnVS gene (oCnVS); growth curves of C. glutamicum cultures in BHI complex and CGXII minimal media (Fig. S1); basal expression background of cIPTG-supplemented cultures in BHI and CGXII media in the dark (Fig. S2); dynamic range of induction for IPTG- and cIPTG-based inductions after 3 and 20 h of expression in BHI and CGXII media, using C. glutamicum ATCC 13032(pEKEx-2-EYFP) (Fig. S3); propidium iodide-based live/dead staining using flow cytometric single-cell analysis to evaluate cell viability (Fig. S4); reduction of growth impairment during (+)-valencene production in VLC6 via delayed induction and application of cIPTG-based light induction (Fig. S5); growth analysis of C. glutamicum VLC6 during IPTG- and cIPTG-induced (+)-valencene production for different induction time points (Fig. S6); optimization of IPTG concentrations for C. glutamicum VLC6 production cultures induced after 4 h of cultivation (Fig. S7); summary of titers as well as volumetric and biomass-specific productivities for the conducted (+)-valencene production in different C. glutamicum strains using CGXII minimal medium (Table S1).
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