Glucose or Altered Ceramide Biosynthesis Mediate Oxygen Deprivation Sensitivity Through Novel Pathways Revealed by Transcriptome Analysis in Caenorhabditis elegans

Supplemental Material for Ladage et al., 2016

Files in this Data Supplement:

  • Figure S1 - RNA-Seq analysis reveals differentially regulated genes in hyl-2(tm2031) animals. (.jpg, 109 KB)
  • Figure S2 - Transcripts were categorized based on Gene Ontology (GO) annotations for biological functions; the number of genes for each category is displayed in the pie-chart. (.jpg, 737 KB)
  • Figure S3 - A heatmap showing the transcriptional profile for genes differentially expressed in N2 animals fed a glucose diet and in hyl-2(tm2031) mutants. (.jpg, 488 KB)
  • Figure S4 - Relative to N2 control, RNAi of the indicated genes did not significantly increase anoxia survival. (.jpg, 53 KB)
  • Figure S5 - Validation of RNA-Seq analysis by qRT-PCR for cyp-25A1 and ugt-63 transcripts that showed downregulation in animals fed a glucose diet and the hyl-2(tm2031) mutant relative to wild-type N2 animals fed a standard OP50 diet. (.jpg, 76 KB)
  • Table S1 - Upregulated transcripts in hyl-2 animals. (.xlsx, 177 KB)
  • Table S2 - Upregulated transcripts in hyl-2 animals fed a glucose diet. (.xlsx, 797 KB)
  • Table S3 - Transcripts upregulated in N2 and hyl-2 animals fed a glucose diet. (.xlsx, 209 KB)
  • Table S4 - Transcripts downregulated in N2 and hyl-2 animals fed a glucose diet. (.xlsx, 160 KB)
  • Table S5 - Lipid metabolism gene datasets. (.xlsx, 41 KB)
  • Table S6 - Common upregulated transcripts in anoxia sensitive animals (N2 glucose and hyl-2(tm2031)). (.xls, 425 KB)
  • Table S7 - Babelomics analysis of the 199 common transcripts. (.xlsx, 30 KB)
  • Table S8 - Cluster 1 transcripts. (.xlsx, 67 KB)