CLUSTAL multiple sequence alignment by MUSCLE (3.8) Dmojavensis1_261 --------MTFSHWYRDREVESQQ------------------------------------ Dmelanogaster1_266 --------MTITNWFNWSVKDLSN------------------------------------ Dyakuba1_267 --------MTITNWYNWSVKDFSN------------------------------------ Dmojavensis3_375 -MANISNNEYKKTDFDWNEISVGDYNLDHSQNFFALASTSNKE----------------N Dyakuba3_319 --MDNANSTFDKSEFDWDAINVGDYQLDHSQNFFARANKENNR----------------G Dmelanogaster3_325 --MDNASSTFGQSDFDWDAINVGDYQLDHSQNFFARANKENNR----------------S Cquinquefasciatus_257 ----MERQQLEDEDDDDGVISCQS------------------------------------ Aaegypti_251 ------------MEFDWDNISIGEPALNTSNSYF-------------------------- Dmojavensis2_289 -----------MSELKFDE-KCSEFDLESTEELFDRMN---------------------- Dyakuba2_276 -------MSDSKAEYNWGDLGMEP------------------------------------ Dmelanogaster2_275 -------MSDSKAEYNWGDLGVES------------------------------------ Ccapitata_397 ------MSYLMPNDFNWDDIDVGNHVLDHSQKFFQQKHNTHERLDESKEMDLIKEIDAIS Mdomestica_331 MAYYNVKMPEVQVKFDWDDIDVGNDQLDHSENFFAIKHYEHER----------------- Dmojavensis1_261 -----------EKPKSKLVNRV----------QRLDARLQSIVLENNTNR---------- Dmelanogaster1_266 ----------ESHARPKLIPGF----------DQLDKRLQAALQERDVQKHQ-------- Dyakuba1_267 ----------ESHARPKLIPGF----------DQLDERLKAALQERDLVKDQ-------- Dmojavensis3_375 MGD----QLNWNNEKMTREQQDRSVCDQNLNDDDIVKLNTSELLRENALQDKPVCDQNLN Dyakuba3_319 LSNVNPLQLCPTPQKICREQRDGDFGGKLVCAEDLIKESPKYVIE--APKDAPTIESKLE Dmelanogaster3_325 LSNVNPLLLCTTPQKIGREQRDGDFDGKLISAGDLKKESPKDTTE--ASKDALTIKPKVE Cquinquefasciatus_257 -----------SEPDPSVAAG--------------------------------------- Aaegypti_251 ------------FRDIKMEKGD---EPNLDCAASSSSSGNSARIHHPDDK---------- Dmojavensis2_289 -------LQEQDEENLSFEDDESTLKQHQLDQDLEQVQRRQSIVFENAQKQVAVLE---- Dyakuba2_276 --------MDSFASEIRTSGEFPSLQQHREQVEQLQRRGRQ-LVSQAQQHSQ-------- Dmelanogaster2_275 ----------MESFASAISEEFPRLQQHREQVEQLQRRGRQ-IVSQAQQHSQ-------- Ccapitata_397 LDDTFESSQNKENIDVCSKATETNSPLKELSITALNDGNKTNLQSPKNEPKINPGFHNTK Mdomestica_331 -------LEDIGLLEEEMAKTELTDIEEQCVIYKIEKHDNTITAEEEEEKEYDKEDKENK Dmojavensis1_261 ------------------------------------------------------------ Dmelanogaster1_266 ------------------------------------------------------------ Dyakuba1_267 ------------------------------------------------------------ Dmojavensis3_375 VEDIVKLRTIERLGEDGPEDKSACDPYLNEGNTSDGKAEQEEKEDQSASYQNLNEDYIVH Dyakuba3_319 PQDMPKVEPKAEL-------PVEYDP----------EPQDDAKPIVPLNWGTNGDDDEKH Dmelanogaster3_325 PHDVPELKLKTELQELAQVSPLDNGP----------EPQDDIKQIVPLNWGKDGDDDEKH Cquinquefasciatus_257 ------------------------------------------------------------ Aaegypti_251 ------------------------------------------------------------ Dmojavensis2_289 ------------------------------------------------------------ Dyakuba2_276 ------------------------------------------------------------ Dmelanogaster2_275 ------------------------------------------------------------ Ccapitata_397 NCDDSSVRAPLSEQQTNATNALESLIARNKARINPSVNKCKTDPESNIDDAHTKAANPTV Mdomestica_331 DSSINSSQNEEEV-----------------------KSPADVKPQNHTDNGHQESHLDKT Dmojavensis1_261 ------------------------------------------------LLRQQAGLDK-- Dmelanogaster1_266 ------------------------------------------------RKRTQAGGGQ-- Dyakuba1_267 ------------------------------------------------RKRKQAGGGQ-- Dmojavensis3_375 IESELQLQLQLDLSTDSHQAEGDKVESLPIPKQAESISDQEMEVNEILGLKPRLKLNW-- Dyakuba3_319 MTEADQHLIHQDARHP--------------------------------AVGGKLRLEW-- Dmelanogaster3_325 MTEADQHLVHQE-RPP--------------------------------GVGAKLRLEW-- Cquinquefasciatus_257 ------------------------------------------------PSKSRAKLDQ-- Aaegypti_251 ------------------------------------------------ALLEEYQRHQ-- Dmojavensis2_289 ------------------------------------------------NLLKTVEPDW-- Dyakuba2_276 ------------------------------------------------MLVANLFKSL-- Dmelanogaster2_275 ------------------------------------------------VLVSNLFKNM-- Ccapitata_397 FSESLIKRNKA-------------------------------------RINPGVKKENII Mdomestica_331 VQQTNL------------------------------------------APKLNLVCQWST Dmojavensis1_261 ----ARMNSKKKKKTLRG-------SPKVCSIQHVYDRNRQQELRQLQQEQSRQCQFHSH Dmelanogaster1_266 ----SQAKPKKLKTTRKQ-------TPRVCKFQKVYTEKRKKLKREANMQ-SNAFQFRSR Dyakuba1_267 ----SQRKPKKLKRTRGQ-------TPRVCEFQQAYTEKRRELKRLANTQ-RNAFQFRSR Dmojavensis3_375 ----TPKPKNQSEPVSAGK------APRVYECKDVYQMKREMIMRQREEEERKAREFHSK Dyakuba3_319 ----SPLSHRELEGAGASAASATTTAPKAYQFKDVYESKRQQLMRRRSEEESKARQFHSR Dmelanogaster3_325 ----SPPRHREHEGAGASATSA---APKAYQFKDVYESKRQQAMRKRSEEESKARQFHSR Cquinquefasciatus_257 ----AKIQQQEA-------------CNRAYKYAQVYEKHRKALREKEERQLQEMKKFQAR Aaegypti_251 ----AKTRQGET-------------CDRAYRYAQVYENKRKALREKEEKQLQEMKKFHAR Dmojavensis2_289 ----QKAVEKRMTAGECP-------PPQAYRFKDIYERKKQQLRQQHLAEERAKRQFHSR Dyakuba2_276 ----KLGSERNTLPTQVS-------TPKAYRFKDIYSSRKDQLIRECQEQERKQREFHSR Dmelanogaster2_275 ----KISCEKPTFPPQVS-------APKAYRFKDIYSSRKDQLIRECREQERKQREFHSR Ccapitata_397 KPNASPTTSGNAVSKGAVPKQPASVAPHAYHCSELYKRRKEEQLQKRLEEERKMREFHSR Mdomestica_331 QKPATKKCHTQAATSKGAVSKSMPPPPQAYLVKDLYQKKREEAERRREEEERKRREFHSR .. * .. : :*.:. Dmojavensis1_261 AVPNF------KLMHKRWEIRNRMRQLKCLYNLTKPQTPHTLKKSM-EAAKRWQTERANL Dmelanogaster1_266 PVPDF------ERSHRR--LERRKLYLNSLQTVTKPRCPATLATSM-VALNKRLKKQRQS Dyakuba1_267 PVPDF------ERSHRQ--LERRRLYLNSLQKLTKPRGPGTFSASMKPSANSQPKKRIQC Dmojavensis3_375 PVPNF------KALHKR--LADTIV----THKITVPVTPETLKHSY-EHLQRRKSREEEH Dyakuba3_319 PMPNF------RAMHKR--MGELAV----IHKITIPRTPETLKHSQMNAERRREDDQAR- Dmelanogaster3_325 PMPNF------KAMHKR--MNELAV----IHKITVPRTPETLKHGQSNVERRRDDDPADQ Cquinquefasciatus_257 PAPNFTRKEPKDTAQGY--GKKENV----EPKFTIPVTPKQMKPERLKKAADQSKKLQDR Aaegypti_251 PAPNF------KSGSKK--ENA-------LPKFTIPVTPKQMKPERIKKHALQVKKLEDR Dmojavensis2_289 PMPNF------SRAHRY--LANRPT----VHRITCAVTPNVLKTSR-QMLLKRKQKIEQM Dyakuba2_276 PMPDF------RQAHER--QASKVI----VHRVTCPTTPNVLKNSR-DMEQKRRLHVQQV Dmelanogaster2_275 PMPDF------RQAHSR--QSSRVV----VHRITCPTTPNVLKNSR-EMEEKRRLRVEQL Ccapitata_397 PVPNF------TACHKS--LQDKKL----LHVVTVAVTPQVLKKSR-ESEEKRKQKLDQI Mdomestica_331 PVPNF------NACHQL--MQKRKP----SHAFTVAVTPKVLKKSK-EAEEKRKQRLEEW . *:* .* . * : . Dmojavensis1_261 EQQIMSEVTL---RPQLSQASKNWRQPPFVPRILSSIV------KTEPFHLKSVERGKLR Dmelanogaster1_266 RKTSDFVP-----RINPGSSMDYLNRQPFTPLVKSSFT------HPKPFHLHTSERALRR Dyakuba1_267 PKASDFVP-----RINPGSSMDYLNRQPFVPHVKSSYT------HPKPFRLHTSDRALSR Dmojavensis3_375 DNARN--------------------------LRRAGLA------EVKPFQLRADQRVRER Dyakuba3_319 --------------------------------LHAVGT------RSKPFHLRSEQRVRDR Dmelanogaster3_325 AR------------------------------LHPAGT------RSRPFHLRSEQRVRDR Cquinquefasciatus_257 TKPAEPS------KEFKAHDARVLKEAPFQPVLAKTVV------KQDPFNLRMSARLAER Aaegypti_251 TKEPEPS------KEFKANDAKVLKEEPFKPALVKTVI------KSAPFELRMSSRLQER Dmojavensis2_289 QREQEELQKVN--MYPKPKPVPKFKPAPLKPANAPLQL------EVKPFRLSTELRAEQR Dyakuba2_276 QREREQQCQLHRMQAPRAKPIPLSSRQPLRSSNRPAAA-PPLKLKVEPFNLSAELRVQQR Dmelanogaster2_275 QRERELESQLHKMQAPRAKPIPQSSRQPLKSSNRPSSAVPAARVKVEPFNLSAESRVQQR Ccapitata_397 KQVLP--------PKFEPRPPNILHEKPFIPKKTQTVL------VSCPFKLNSERRLQER Mdomestica_331 KQKSQA-------PKFESRPPTVLKEKPFVPEKKPLVI------QQCPFNLHTEKRLAER ** * * * Dmojavensis1_261 KQFDLWNRREQETRWQRKAIEWAQRCHDEYVQARKLTNFKATPNPWKRTKAII------- Dmelanogaster1_266 QVYDERKRIRMDRRLEQQTIDWFARERSEYFKLRKMTNFKATPNPWKQTRVTARKDSQAA Dyakuba1_267 QLYDERKRIRMDQRLDQLARDWFARERSEYFRLRKMTNFQATPNPWKRTKLTTKTVSQGA Dmojavensis3_375 REYNNVVQCNLDEKKKQRLIVALAAAIQQMRRN--------------------------- Dyakuba3_319 REFDAAVQASLEQKKKEVKTNSSSH----------------------------------- Dmelanogaster3_325 REFDAAVQVSLEQKKKEVKTKSSSP----------------------------------- Cquinquefasciatus_257 RQFDEQLQRAKEEKARRDAEEKRLAEERELKLLRKQKEFKANPNPFKFTKLVELSYCSCD Aaegypti_251 KQIDEQRQRAKEEKARREAEEKRLAEERELKLLRKQKEFKANPNPFNH------------ Dmojavensis2_289 KIFNAQTLQAQEAKRRQLEEQRKRREEEEFQKQRQLATFRARPNPFKYQFGGSATN---- Dyakuba2_276 RLFNIQTSKAQEARRRQLEDQRQRAEREAYQKQRQRTTFRARPNPFSQTAR--------- Dmelanogaster2_275 RIFNIQTSKAQEARRRELEDQRQRAEREAYQKLRQRTTFRARPNPFSQTAR--------- Ccapitata_397 KQYDATVQRAMEEKQKQEEEERKRREELRIKELRKLTKFKARPNPFK------------- Mdomestica_331 KHYDEAKHKALEEKRKQVEIFMILSI---------------------------------- . : : . Dmojavensis1_261 ------------ Dmelanogaster1_266 ------------ Dyakuba1_267 ------------ Dmojavensis3_375 ------------ Dyakuba3_319 ------------ Dmelanogaster3_325 ------------ Cquinquefasciatus_257 ESQSFPIIHGLT Aaegypti_251 ------------ Dmojavensis2_289 ------------ Dyakuba2_276 ------------ Dmelanogaster2_275 ------------ Ccapitata_397 ------------ Mdomestica_331 ------------