Top 4 BLAST hits comparing the putative and confirmed P450s encoded in the genome of S. yanoikuyae strain B2 to proteins in the UniProt database (Table S1); pairwise sequence alignment of characterized members of the CYP101 family and CYP176A1 with the three new 1,8-cineole-hydroxylating P450s from S. yanoikuyae strain B2 using BLASTp, showing alignment length/identities (Table S2); overview of the S. yanoikuyae strains for which a whole-genome shotgun project is available at DDBJ/ENA/GenBank (Table S3); relatedness of the six sequenced S. yanoikuyae strains and an outgroup taxon, Sphingobium indicum B90A, calculated by counting the number of 25-mers shared between each pair of organisms (Table S4); multiple sequence alignment of CYP101J2, CYP101J3, and CYP101J4 with previously characterized members of the CYP101 family and CYP176A (Fig. S1); phylogenetic relationship between selected strains in the genus Sphingobium ((Fig. S2); additional experimental details (production of S. yanoikuyae B2 cells, purification of proteins from S. yanoikuyae B2, partial sequencing of 1,8-cineole-binding P450s).
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