Multisequence alignment of amino acid sequences containing the domain of unknown function DUF2236 (Fig. S1); UV-vis spectra of LcpK30 wild type (from Streptomyces sp. K30), LcpRr (from R. rhodochrous PRK1), and the LcpK30 muteins purified in this study (Fig. S2); oxygen consumption assays during polyisoprene cleavage (Fig. S3); HPLC chromatograms of LcpK30-generated cleavage products (Fig. S4); list of amino acid sequence identifiers used for the multiple sequence alignments (Table S5).
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