This PDF file includes:

• fig. S1. Helicase activity is essential for processive replication by the T7 replisome.
• fig. S2. Strong nucleosomal barrier affects the processivity of the T7 replisome.
• fig. S3. Analysis of the nucleosomal pausing patterns formed during replication by exo⁺ and exo⁻ replisomes.
• fig. S4. Analysis of time courses of replication through chromatin by the exo⁺ (A) or exo⁻ replisome (B) using the KinTek Explorer software.
• fig. S5. Mapping of nucleosome positions after replication by the T7 replisome using restriction enzyme sensitivity assay.
• fig. S6. Nucleosomes are not formed de novo during or after T7 replication.
• fig. S7. Proposed role for exonuclease activity during replication through a nucleosome.
• table S1. Sequences of oligonucleotides and DNA templates.

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