This PDF file includes:

• fig. S1. In vivo assessment of modality responses following NaV1.8 deletion.
• Legends for movies S1 to S8

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.avi format). Neuronal activation assessed by GCaMP6s fluorescence in vitro.
• movie S2 (.avi format). Frequency-dependent (0.5 Hz) changes in neuronal activation assessed by GCaMP3 fluorescence.
• movie S3 (.avi format). Frequency-dependent (1 Hz) changes in neuronal activation assessed by GCaMP3 fluorescence.
• movie S4 (.avi format). Frequency-dependent (10 Hz) changes in neuronal activation assessed by GCaMP3 fluorescence.
• movie S5 (.avi format). Neuronal activation assessed by GCaMP3 fluorescence in vivo.
• movie S6 (.avi format). Neuronal activation following FCA treatment assessed by GCaMP3 fluorescence in vivo.
• movie S7 (.avi format). Neuronal activation before PGE₂ treatment assessed by GCaMP3 fluorescence in vivo.
• movie S8 (.avi format). Neuronal activation following PGE₂ treatment assessed by GCaMP3 fluorescence in vivo.