Conserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome

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  Fig. S1. (A) Viability and ribosome depurination in yeast expressing wild-type (WT) or mutant Stx1A1 or Stx2A1. (B) Depurination of yeast ribosomes. Fig. S2 (A) Translation inhibition in mammalian cells by WT Stx1A1 or Stx2A1 or Stx1A1 and Stx2A1 variants. (B) Shiga toxin gene expression in Vero cells. (C) Depurination of ribosomes in mammalian cells expressing WT or mutant forms of Stx1A1 and Stx2A1. Table S1. EGFP fluorescence statistical significance of the contrasts. Table S2. Depurination statistical significance of the contrasts.

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